Adipogen/X-Gal/AG-CC1-0003-M250/250 mg-免疫在线蚂蚁淘旗下平台-

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Adipogen/X-Gal/AG-CC1-0003-M250/250 mg

来自 : 发布时间：2025-11-07

More Information Product Details Synonyms Product Type Properties Formula MW CAS Purity Chemicals Appearance Solubility Identity Other Product Data InChi Key Shipping and Handling Shipping Short Term Storage Long Term Storage Handling Advice Use/Stability Documents MSDS Product Specification Sheet Datasheet

5-Bromo-4-chloro-3-indolyl β-D-galactoside

Chemical

C₁₄H₁₅BrClNO₆

408.6

7240-90-6

≥ 99% (HPLC)

White to off-white powder. Clear, colourless to light yellow in solution.

Soluble in dimethyl formamide.

Determined by IR.

Optical Rotation [α]D20 (c=1,DMF:water, 1:1): -61.5° ± 1.0°Water Content (Karl Fischer): max 1%.

OPIFSICVWOWJMJ-LMNFNBGQSA-N

AMBIENT

+4°C

-20°C

Protect from light and moisture.

Stable for at least 3 years after receipt when stored at -20°C.

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Molecular biology reagent.
Chromogenic substrate for β-galactosidase.
Used in gene cloning together with IPTG (Prod. No. AG-CC1-0002) to indicate whether a cell expresses the β-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening.
Distinguishes between recombinant and non-recombinant plasmids carrying the β-galactosidase gene.
Used in two-hybrid analysis.
X-Gal is used to determine E. coli and coliform content in drinking water samples.

Product References

Chemiluminescent assay of various enzymes using indoxyl derivatives as substrate and its applications to enzyme immunoassay and DNA probe assay: H. Arakawa, et al.; Anal. Biochem. 199, 238 (1991)

In situ histochemical detection of beta-galactosidase activity in lung: assessment of X-Gal reagent in distinguishing lacZ gene expression and endogenous beta-galactosidase activity: D.J. Weiss, et al.; Hum. Gene Ther. 8, 1545 (1997)

A novel colorimetric yeast bioassay for detecting trichothecene mycotoxins: K.H. Engler, et al.; J. Microbiol. Methods 35, 207 (1999)

Molecular Cloning: A Laboratory Manual 3rd Edition: J. Sambrook & D. Russell; Cold Spring Harbor Laboratory Press (2001)

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发布于： 2025-11-07 阅读（）

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