| ProductDetails | |
|---|---|
| Synonyms | VascularEndothelialGrowthFactorReceptor1;FLT-1;Fms-likeTyrosineKinase1 |
| ProductType | Protein |
| Properties | |
| Source/Host | Sf9cells |
| Sequence | HumansVEGFR-1(663aa). |
| Crossreactivity | Human |
| BIOLOGicalActivity | TheactivityofsVEGFR-1wasdeterminedbyitsABIlitytoinhibittheVEGF-A-inducedproliferationofHUVECs. |
| MW | ~96kDa(monomer) |
| Purity | ≥95%(SDS-PAGE) |
| Reconstitution | Solubleinwaterandmostaqueousbuffers.ReconstitutewithPBStoaconcentrationnotlowerthan100ng/ml. |
| Formulation | Lyophilized. |
| OtherProductData | UniProtlinkP17948-2:sVEGFR-1(human) |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Avoidfreeze/thawcycles. |
| Use/Stability | Afterreconstitution,storeat-80°C. Stableforatleast6monthsafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Recombinanthumansolublevascularendothelialgrowthfactorreceptor-1(sVEGFR-1)isthenaturallyoccurringformandisaglycosylatedmonomericprotein.ThebiologicalfunctionofsVEGFR-1seemstobeanendogenousregulatorofangiogenesis,bindingVEGFwiththesameaffinityasthefull-lengthreceptor.VEGFR-1isatyrosine-proteinkinasethatactsasacell-surfacereceptorforVEGFA,VEGFBandPGF,andplaysanessentialroleinthedevelopmentofembryonicvasculature,theregulationofangiogenesis,cellsurvival,cellmigration,macrophagefunction,chemotaxisandcancercellinvasion.Itmayplayanessentialroleasanegativeregulatorofembryonicangiogenesisbyinhibitingexcessiveproliferationofendothelialcells.Itcanpromoteendothelialcellproliferation,survivalandangiogenesisinadulthood.Itsfunctioninpromotingcellproliferationseemstobecell-typespecific.PromotesPGF-mediatedproliferationofendothelialcells,proliferationofsometypesofcancercells,butdoesnotpromoteproliferationofnormalfibroblasts(invitro).IthasaveryhighaffinityforVEGFAandrelativelylowproteinkinaseactivity.ItmayfunctionasanegativeregulatorofVEGFAsignalingbylimitingtheamountoffreeVEGFAandpreventingitsbindingtoKDR.ModulatesKDRsignalingbyformingheterodimerswithKDR.Ligandbindingleadstotheactivationofseveralsignalingcascades.ActivationofphospholipaseC-γ(PLCG)leadstotheproductionofthecellularsignalingmoleculesdiacylglycerolandinositol1,4,5-trisphosphateandtheactivationofproteinkinaseC.MediatesphosphorylationofPIK3R1,theregulatorysubunitofphosphatidylinositol3-kinase,leADIngtoactivationofphosphatidylinositolkinaseandthedownstreamsignalingpathway.MediatesactivationofMAPK1/ERK2,MAPK3/ERK1andtheMAPkinasesignalingpathway,aswellasoftheAKT1signalingpathway.PhosphorylatesSRCandYES1andmayalsophosphorylateCBL.
- SolubleVEGFR-1secretedbyendothelialcellsandmonocytesispresentinhumanserumandplasmafromhealthydonors:B.Barleon,etal.;Angiogenesis4,143(2001)
- Poorresponder-highresponder:theimportanceofsolublevascularendothelialgrowthfactorreceptor1inovarianstimulationprotocols:J.Neulen,etal.;HumanReprod.16,621(2001)
- ActivationofVascularEndothelialGrowthFactorReceptor-1SustainsAngiogenesisandBcl-2ExpressionViathePhosphatidylinositol3-KinasePathwayinEndothelialCells:J.Cai,etal.;Diabetes52,2959(2003)
- ElevatedPlacentalSolubleVascularEndothelialGrowthFactorReceptor-1InhibitsAngiogenesisinPreeclampsia:S.Ahmad&A.Ahmed;Circ.Res.95,884(2004)
- AutocrineactivityofsolubleFlt-1controlsendothelialcellfunctionandangiogenesis:S.Ahmad,etal.;Vasc.Cell3,15(2011)
- TheroleofheterodimerizationbetweenVEGFR-1andVEGFR-2intheregulationofendothelialcellhomeostasis:MJ.Cudmore,etal.;Nat.Commun.3,972(2012)
- AlteredredoxstatemodulatesendothelialKCa2.3andKCa3.1levelsinnormalpregnancyandpreeclampsia:S.Choi,etal.;Antioxid.Redox.Signal,aheadofprint(2018)
