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Adipogen/SUMO E1 (human) (rec.)/AG-40T-0359-C025/25 µg
Adipogen/SUMO E1 (human) (rec.)/AG-40T-0359-C025/25 µg
商品编号：
AG-40T-0359-C025
品牌：
Adipogen Inc
市场价：
¥7320.00
美元价：
4392.00
产品分类：
其它重组蛋白
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductDetails | |
|---|---|
| Synonyms | SUMO1ActivatingEnzymeE1;SUA1;SAE1;UBA2;UBLE1A;Ubiquitin-like1-activatingenzymeE1A |
| ProductType | Protein |
| Properties | |
| Source/Host | E.coli |
| Sequence | HumanSUMOE1(AccessionNr.Q9UBE0.1). |
| Crossreactivity | Human |
| MW | ~110kDa |
| Purity | ≥90%(SDS-PAGE) |
| Formulation | Liquid.In50mMHEPESpH8,200mMNaCl,1mMDTTand10%Glycerol. |
| OtherProductData | Use:MemberoftheSUMO-activating(E1)enzymefamilythatisrequiredforthefirststepoftheenzymaticcascadethatsubsequentlyutilizesaSUMO-conjugating(E2)enzymetoconjugateSUMOtosubstrateproteins.ASUMOligase(E3)issometimesutilizedforSUMOconjugation,butisnotalwaysrequired.Reactionconditionswillneedtobeoptimizedforeachspecificapplication.Werecommendaninitialproteinconcentrationof50-500nM. |
| Declaration | ManufacturedbyBostonBiochem |
| ShippingandHandling | |
| Shipping | DRYICE |
| ShortTermStorage | -20°C |
| LongTermStorage | -80°C |
| HandlingAdvice | Aliquottoavoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-80°C. |
| Documents | |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet |  DownloadPDF |
SUMOActivatingEnzymeSubunit1(SAE1)isthehighlyconservedhumanorthologofyeastubiquitin-activatingenzymeE1-like(UBA2).TheseSUMO-activating(E1)enzymesarecriticalfortheenzymaticattachmentofSUMOmoleculestoatargetproteinbyapost-translationalmodificationprocesstermedSUMOylation.TheATP-dependentE1enzymechargestheSUMObyformingahigh-energythiolesterintermediatewhichistransferredtotheUBE2I/Ubc9SUMO-conjugating(E2)enzyme.Thesecondstepisthetrans-esterificationreactionwherebySUMOistransferredtoCys93ofUbcH9.UBE2I/Ubc9istheonlyknownE2thatisabletomediatetheconjugationofSUMOtolysineresiduesonavarietyofcellulartargets,usuallyintheabsenceofaubiquitinligase(E3).AlthoughUBE2I/Ubc9candirectlyrecognizeandmodifylysineresiduescontainedinaSUMOylationmotif,E3-likefactorsmostlikelyfacilitatetheSUMOylationofspecificsubstrates.Conjugationoftheubiquitin-likemodifierSUMOrequirestheactivitiesoftheheterodimericE1(Aos1/Uba2)andtheUbcH9E2enzyme.ThedimericactivatingenzymeutilizesATPtoadenylatetheC-terminalglycineresidueofSUMO-1(alsoSUMO-2andSUMO-3),formingahigh-energythiolesterbondwiththecysteineresidueofUba2andthereleaseofAMPandPPi.Thesecondstepisthetrans-esterificationreactionwherebySUMO-1istransferredtoCys93ofUbcH9.
品牌介绍
Adipogen公司由三个子公司组成，分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国，运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发，如癌症、免疫、炎症、代谢疾病（糖尿病、肥胖）、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外，也可提供抗体和蛋白产品，Adipogen拥有自己的化学实验室，致力于开发创新型小分子化合物。
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