| ProductDetails | |
|---|---|
| Synonyms | Proepithelin;PEPI;PCCell-derivedGrowthFactor |
| ProductType | Protein |
| Properties | |
| Source/Host | HEK293cells |
| Sequence | Signalpeptideandmouseprogranulin(aa1-589)isuntagged.Reflectsthenativesequencewithnoadditionalaa. |
| Crossreactivity | Mouse |
| MW | ~70kDa(SDS-PAGE) |
| Purity | ≥95%(SDS-PAGE) |
| EndotoxinContent | <0.01eu mu;g="" purified="" protein="" (lal="" test;="" lonza).=""> |
| Concentration | Afterreconstitution: for10µgsize:0.1mg/ml for50µgsize:1mg/ml |
| Reconstitution | 10µgsize:Reconstitutewith100µlsterilewater. 50µgsize:Reconstitutewith50µlsterilewater. |
| Formulation | Lyophilized.ContainsPBS. |
| OtherProductData | UniProtlinkP28798:Granulins(mouse)[Precursor] |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Afterreconstitution,preparealiquotsandstoreat-20°C. Avoidfreeze/thawcycles. PBScontainingatleast0.1%BSAshouldbeusedforfurtherdilutions. |
| Use/StABIlity | Stableforatleast6monthsafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Progranulin(PGRN)isawidelyexpressedpluripotentgrowthfactorwhichplaysaroleinprocessessuchasdevelopment,woundrepairandinflammationbyactivatingsignalingcascadesthatcontrolcellcycleprogressionandcellmotility.Itsfunctioninthecentralnervoussystemisofinterest,asmutationsinthePGRNgenewerefoundincasesoffrontotemporaldegeneration(FTLD).Inaddition,PGRNhasalsobeenlinkedtotumOrigenesis.ProgranulinisabioMarkerforFTLD,othertypesofAlzheimer‘sDisease(AD)andpotentiallyforMCI(MildCognitiveImpairment).Additionally,PGRNisdescribedasanewligandofTNFreceptorsandapotentialtherapeuticagainstinflammatorydiseaselikearthritis.EphA2,amemberofthelargefamilyofEphrinreceptortyrosinekinases,isalsoafunctionalsignalingreceptorforprogranulinasreportedrecently.
- Progranulinpromotestumournecrosisfactor-inducedproliferationofsuppressivemouseCD4⁺Foxp3⁺regulatoryTcells:Y.Hu,etal.;Immunol.142,193(2014)
- Progranulinandafivetransmembranedomain-containingreceptor-likegenearethekeycomponentsinreceptoractivatorofnuclearfactorB(RANK)-dependentformationofmultinucleatedosteoclasts:J.Oh,etal.;J.Biol.Chem.290,2042(2015)
- Progranulinregulateslysosomalfunctionandbiogenesisthroughacidificationoflysosomes:Y.Tanaka,etal.;Hum.Mol.Genet.aheadofprint2017
