| ProductDetails | |
|---|---|
| Synonyms | Proepithelin;PEPI;PCCell-derivedGrowthFactor |
| ProductType | Protein |
| Properties | |
| Source/Host | HEK293cells |
| Sequence | Signalpeptideandhumanprogranulin(aa1-593)arefusedattheC-terminustoaFLAG®-tag. |
| Crossreactivity | Human |
| BIOLOGicalActivity | ActivatesERKphosphorylationinTHP-1andMCF10AcellsandinducescellmigrationofTHP-1monocyticleukemiacells. |
| MW | ~76kDa(SDS-PAGE) |
| Purity | ≥98%(SDS-PAGE) |
| EndotoxinContent | <0.01eu mu;g="" purified="" protein="" (lal="" test;="" lonza).=""> |
| Concentration | Afterreconstitution: for10µgsize:0.1mg/ml for50µgsize:1mg/ml |
| Reconstitution | 10µgsize:Reconstitutewith100µlsterilewater. 50µgsize:Reconstitutewith50µlsterilewater. |
| Formulation | Lyophilized.ContainsPBS. |
| OtherProductData | UniProtlinkP28799:Granulins(human)[Precursor] FLAGisaregisteredtrademarkofSigma-AldrichCo. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Afterreconstitution,preparealiquotsandstoreat-20°C. Avoidfreeze/thawcycles. PBScontainingatleast0.1%BSAshouldbeusedforfurtherdilutions. |
| Use/StABIlity | Stableforatleast6monthsafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Progranulin(PGRN)isawidelyexpressedpluripotentgrowthfactorwhichplaysaroleinprocessessuchasdevelopment,woundrepairandinflammationbyactivatingsignalingcascadesthatcontrolcellcycleprogressionandcellmotility.Itsfunctioninthecentralnervoussystemisofinterest,asmutationsinthePGRNgenewerefoundincasesoffrontotemporaldegeneration(FTLD).Inaddition,PGRNhasalsobeenlinkedtotumOrigenesis.ProgranulinisabioMarkerforFTLD,othertypesofAlzheimer‘sDisease(AD)andpotentiallyforMCI(MildCognitiveImpairment).Additionally,PGRNisdescribedasanewligandofTNFreceptorsandapotentialtherapeuticagainstinflammatorydiseaselikearthritis.EphA2,amemberofthelargefamilyofEphrinreceptortyrosinekinases,isalsoafunctionalsignalingreceptorforprogranulinasreportedrecently.
