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Adipogen/IL-33 (oxidation resistant) (human) (rec.) (His)/AG-40B-0167-C100/100 µg
| ProductDetails | |
|---|---|
| Synonyms | IL-33(human)(C208S/C227S/C232S/C259SMutant);Interleukin-33(human)(C208S/C227S/C232S/C259SMutant);IL-1F11;NF-HEV |
| ProductType | Protein |
| Properties | |
| Source/Host | E.coli |
| Sequence | HumanIL-33(aa112-270)isfusedattheC-terminustoaHis-tag.AminoacidsC208,C227,C232andC259havebeenmutatedtoserinetoprotectIL-33fromoxidation. |
| Crossreactivity | Human Mouse |
| Specificity | BindstohumanandmouseST2. |
| BIOLOGicalActivity | ActivateshumanandmouseST2-dependentNF-κBpathway. |
| MW | ~17kDa |
| Purity | ≥95%(SDS-PAGE) |
| EndotoxinContent | <0.01eu mu;g="" purified="" protein="" (lal="" test;="" lonza).=""> |
| Concentration | Afterreconstitution: for10µgsize:0.1mg/ml for100µgsize:1mg/ml |
| Reconstitution | Reconstitutewith100μlsterilewater. |
| Formulation | Lyophilized.ContainsPBS. |
| OtherProductData | UniProtlinkQ2YEJ5:Interleukin-33(human) |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Afterreconstitution,preparealiquotsandstoreat-20°C. Avoidfreeze/thawcycles. PBScontainingatleast0.1%BSAshouldbeusedforfurtherdilutions. |
| Use/StABIlity | Stableforatleast6monthsafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Interleukin-33(IL-33;HF-NEV;IL-1F11),amemberoftheIL-1familyofcytokines,isexpressedbymanycelltypesfollowingpro-inflammatorystimulationandisthoughttobereleaseduponcelllysis.IL-33bindstoandsignalsthroughST2(IL-1R1)anditsstimulationrecruitsMYD88,IRAK,IRAK4andTRAF6,followedbyphosphorylationofERK1(MAPK3)/ERK2(MAPK1),p38(MAPK14)andJNK.TheabilityofIL-33totargetnumerousimmunecelltypes,likeTh2-likecells,mastcellsandB1cells,andtoinducecytokineandchemokineproductionunderlinesitspotentialininfluencingtheoutcomeofawiderangeofdiseases,suchasarthritis,asthma,atopicallergy&anaphylaxis,cardiovasculardisease/atherosclerosis,nervoussystemdiseasesandsepsis.IL-33facilitatesTregexpansioninvitroandinvivo.Recently,IL-33hasbeeninvolvedinADIpocytedifferentiation.ThebiologicalactivityofIL-33atitsreceptorST2israpidlyterminatedintheextracellularenvironmentbyitsoxidation(formationoftwodisulfidebridges),resultinginanextensiveconformationalchangethatdisruptstheST2bindingsite.CysteinesataminoacidsC208,C227,C232andC259controlIL-33oxidationandmutationsoftwoormoreofthesefourcysteinesprotectIL-33fromoxidationandincreaseitsactivity.
- OxidationofthealarminIL-33regulatesST2-dependentinflammation:E.S.Cohen,etal.;Nat.Commun.6,ID8327(2015)
