| ProductDetails | |
|---|---|
| Synonyms | IL-2Superkine(human):Fc(human)(rec.);Interleukin-2;TCellGrowthFactor;TCGF;Aldesleukin;Super-2 |
| ProductType | Protein |
| Properties | |
| Source/Host | HEK293cells |
| Sequence | TheextracellulardomainofhumanIL-2(aa21-153)(mutantH9containingthemutationsL80F/R81D/L85V/I86V/I92F)isfusedattheC-terminustotheFcportionofhumanIgG2. |
| Crossreactivity | Human |
| Application | TriggersfargreaterantitumorresponsesthannativeIL-2invivobutwithlowertoxicity. |
| Specificity | BindstohumanandmouseIL-2R. |
| MW | 42kDa |
| Purity | ≥95%(SDS-PAGE) |
| EndotoxinContent | <0.01eu mu;g="" protein="" (lal="" test;="" lonza).=""> |
| Concentration | 0.1mg/mlafterreconstitution. |
| Reconstitution | Reconstitutewith100μlsterilewater. |
| Formulation | Lyophilized.ContainsPBS. |
| OtherProductData | UniprotlinkP60568: IL-2(human) AfCSSignalingGatewaylinkA006222:IL-2(mouse) |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Afterreconstitution,preparealiquotsandstoreat-20°C. Avoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-20°C. Workingaliquotsarestableforupto3monthswhenstoredat-20°C. |
| Documents | |
| ApplicationNote |  DownloadPDF |
| MSDS | DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Interleukin-2(IL-2)isa133aminoacidglycoproteinwithoneintramoleculardisulfidebondandvariableglycosylation.ItissecretedbyactivatedTcellsandinducesproliferationandmaturationofactivatedTcells,naturalkillercellsandlymphokineactivatedkillercells.IL-2alsostimulatesproliferationofantibody-producingBcells,activatesneutrophilsandinducesmononuclearcellstosecreteIFN-γandTNF-αand-β.Moreover,studieshaveshownthatIL-2isrequiredforactivation-inducedapoptosis,animportanthomeostaticmechanismintheimmunesystem,whichisinvolvedinthemaintenanceofperipheraltolerancetoself-antigens.
IL-2promotesTcellproliferationandparticularlynaiveTcells.IL-2signalingonactivatedTcellsiseffectedthroughaquaternaryhigh-affinityreceptorcomplexconsistingofIL-2,IL-2Rα(CD25),IL-2RβandIL-2Rγ.NaiveTcellsarerelativelyinsensitivetoIL-2astheyonlyexpresssmallamountsofIL-2RβandIL-2Rγ.TheyonlyacquiresensitivityafterCD25expression,whichcapturesthecytokineandpresentsittotheIL-2RβandIL-2Rγreceptors.IL-2Superkine(Fc)isanartificialvariantofIL-2containingmutationsatpositionsL80F/R81D/L85V/I86V/I92F.Thesemutationsarelocatedinthemolecule"scorethatactstostabilizethestructureandtogiveitareceptor-bindingconformationmimickingnativeIL-2boundtoCD25.ThesemutationseffectivelyeliminatethefunctionalrequirementofIL-2forCD25expressionandelicitproliferationofTcells.ComparedtoIL-2,theIL-2superkineinducessuperiorexpansionofcytotoxicTcells,leADIngtoimprovedantitumourresponsesinvivo,andelicitsproportionallylesstoxicitybyloweringtheexpansionofTregulatorycellsandreducingpulmonaryoedema.
- Exploitinganaturalconformationalswitchtoengineeraninterleukin-2"superkine":AM.Levin,etal.;Nature484,529(2012)
