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Adipogen/BAFF, Soluble (human) (60-mer) (rec.) (highly active)/AG-40B-0112-C010/10 µg
| ProductDetails | |
|---|---|
| Synonyms | BLyS;TALL-1;CD257;BCellActivatingFactor;TNFSF13B;TumorNecrosisFactorLigandSuperfamilyMember13B |
| ProductType | Protein |
| Properties | |
| Source/Host | E.coli |
| Sequence | TheextracellulardomainofhumanBAFF(aa134-285)isfusedattheN-terminustoaHis-tag. |
| Crossreactivity | Human Mouse |
| Specificity | BindstohumanandmouseBAFF-R,TACIandBCMA.Relativebindingaffinity(EC50)forBAFF-R(human):~3ng/ml. |
| BIOLOGicalActivity | IncreasesBcellsurvival/proliferation.IncreasesCD21/CD23expressiononBcellsinvivo.ActivatesBAFF-R,TACIandBCMAreceptors.Worksatconcentrations<20ng l.=""> |
| MW | ~19kDa(underreducedconditions) ~1100kDa(undernativeconditions) |
| Purity | ≥95%(SDS-PAGE) |
| EndotoxinContent | <0.01eu mu;g="" purified="" protein="" (lal="" test;="" lonza).=""> |
| Concentration | 0.1mg/mlafterreconstitution. |
| Reconstitution | Reconstitutewith100μlsterilewater. |
| Formulation | Lyophilized.ContainsPBS. |
| OtherProductData | UniProtlinkQ9Y275:BAFF(human) |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Afterreconstitution,preparealiquotsandstoreat-20°C. Avoidfreeze/thawcycles. PBScontainingatleast0.1%BSAshouldbeusedforfurtherdilutions. |
| Use/StABIlity | Stableforatleast6monthsafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
BAFFismainlyproducedbyinnateimmunecellssuchasneutrophils,monocytes,macrophages,dendriticcells,folliculardendriticcells.Tcells,activatedBcells,somemalignantBcellsandalsonon-lymphoidcellslikeastrocytes,synoviocytesandepithelialcellscanalsoproduceBAFF.BAFFbindsthreedistinctreceptors(BAFF-R,TACIandBCMA)expressedpredominantlyonBcells,althoughactivatedTcellsalsoexpressBAFF-R.BAFFisamasterregulatorofperipheralBcellsurvival,andtogetherwithIL-6,promotesIgclass-switchingandplasmacelldifferentiation.BesidesitsmajorroleinBcellbiology,BAFFco-stimulatesactivatedTcells.DeregulatedexpressionofBAFFleadstoautoimmunedisordersinmice.Inhumans,elevatedlevelsofsolubleBAFFhavebeendetectedintheserumofpatientswithvariousautoimmunediseasessuchasSjoegrensyndrome,Rheumatoidarthritis(RA),Multiplesclerosis(MS)andSystemicLupusErythematosus(SLE).BAFFhasalsoincreasedlevelsinsomelymphoidcancers.
ProcessedhumanBAFFcaneitherremainasatrimer,whichisusualforTNFfamilyligandsorassembleinto60-mercomposedof20trimers.MouseBAFF60-merhasbeenidentifiedintheserumofBAFFtransgenicmice.OligomerizationofBAFF3-merinto60-merinhumanBAFFispreventedbymutationofHis218,aresiduecriticalfor3-mer-to-3-merinteractions,butnotforreceptorbinding.DespitethepredominantfunctionalroleofprocessedBAFFinvivo,membrane-boundBAFFmightalsoplayarole.Indeed,solubleBAFF(3-mer)cantriggerBAFF-RbutnotTACIorBCMA,whereasoligomericformsofBAFF(BAFF60-mer),whichmimicmembrane-boundBAFF,activateallBAFFreceptors.
- TACI,unlikeBAFF-R,issolelyactivatedbyoligomericBAFFandAPRILtosupportsurvivalofactivatedBcellsandplasmablasts:C.Bossen,etal.;Blood111,1004(2008)
- MutationoftheBAFFfurincleavagesiteimpairsB-cellhomeostasisandantibodyresponses:C.Bossen,etal.;Eur.J.Immunol.41,787(2011)
- NoevidencethatsolubleTACIinducessignallingviamembrane-expressedBAFFandAPRILinmyeloidcells:J.Nys,etal.;PLoSOne8,e61350(2013)
