>
产品中心 >
Other_reagents >
Adipogen/TLR9 Agonist (human) Explorer Set Endotoxin-free/IAX-300-007-1/1 Set
| ProductDetails | |
|---|---|
| ProductType | Set |
| Properties | |
| ApplicationSet | CompoundScreening |
| KitContains | ODN2216(TypeA)Endotoxin-free(sterile),100µg(Cat.No.:IAX-200-005-C100) |
| OtherProductData | Endotoxinfree:<0.002EU/μg |
| Declaration | ManufacturedbyInnaxon. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | +4°C |
| HandlingAdvice | Avoidfreeze/thawcycles. Afterreconstitution,preparealiquotsandkeepaqueousstocksolutionsfor1dayat4°Corstoreat-20°C(shelf-life6months). |
| Use/StABIlity | Stableforatleast2yearsafterreceiptwhenstoredat+4°C. |
| Documents | |
| Manual | No |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
UnmethylatedCGdinucleotideswithinparticularsequencecontextsareresponsIBLefortheimmunostimulatoryactivityofbacterialDNA.Syntheticoligonucleotides(ODN)thatcontainsuchCpGmotifs(CpGODNs)mimicmicrobialDNA.TheinnateimmunesystemofvertebrateshastheabilitytorecognizeCpGmotifsinmicrobialDNAviatheToll-likereceptor(TLR)9iftheCpGODNwerefreeofadditionalimmunestimulatorycontaminantsoftenpresentinsyntheticcommercialCpGODNpreparationsdesignedformolecularBIOLOGyapplications(i.e.PCR).GiventhathighqualityCpGODNswereused[i.e.endotoxin-free],acloselinkhasbeenestablishedbetweentheexpressionofTLR9oncertainimmunecellsubsetsandthemodulationoftheimmunesystembyCpGDNA.DifferenttypesofCpGODNswereidentifiedbasedontheirdifferingbiologicaleffectsondifferentcelltypes:ODNTypeAisapotentinducerofIFN-αinhumanPDC,(i.e.ODN1585or2216)leADIngtoantigenpresentingcell(APC)maturation,whereasODNTypeB(i.e.ODN2006orODN1668/ODN1826)isaweakinducerofIFN-αbutratherstimulatesIL-8productionandincreasingcostimulatoryandAg-presentingmoleculesandtriggersproliferationofB-cellsandIgMandIL-6production.AthirdtypeofCpGODNhasbeenidentified,termedODNTypeC,withbothhighinductionofINF-αinPDCandactivationofB-cells.ThesequenceofCpGTypeC(alsocalledK)(i.e.ODN2395orM362)combineselementsofbothTypeAandTypeBandcontainsacentralpalindromicsequencewithCGdinucleotides,acharacteristicfeatureofTypeA,andaTCGTCGmotifatthe5"end,presentinTypeBCpGODNs.AlthoughtheCpGmotifsarethoughttodifferbetweenmiceandhumans,inbothspeciestherecognitionofCpGODNsismediatedbyTLR9.TheoptimalCpGmotifinhumansisGTCGTTandGACGTTforthemurinesequence.However,recentevidencesuggeststhatthissequencespecificityisrestrictedtophosphorothioate(PS)-modifiedODNandisnotobservedwhenanaturalphosphodiesterbackboneisused.Inrecentyearssequencerequirements,specificity,signallingpathwaysandkineticsoftheTLR9suppressionbyinhibitoryODNs(iODNs)havebeeninvestigated.
- ComparisonofdifferentCpGoligodeoxynucleotideclassesfortheircapabilitytostimulatehumanNKcells:S.Sivori,etal.;Eur.J.Immunol.36,961(2006)
- InnateimmuneresponsesinducedbyclassesofCpGoligodeoxynucleotidesinovinelymphnodeandbloodmononuclearcells:J.S.Booth,etal.;Vet.Immunol.Immunopathol.115,24(2007)
- ImpactofclassA,BandCCpG-oligodeoxynucleotidesoninvitroactivationofinnateimmunecellsinhumanimmunodeficiencyvirus-1infectedindividuals:J.A.Martinson,etal.;Immunology120,526(2007)
- ThreeCpGoligodeoxynucleotideclassesdifferentiallyenhanceantigen-specifichumoralandcellularimmuneresponsesinmice:Y.Liu,etal.;Vaccine29,5778(2011)
- Toll-likereceptorandIL-12signalingcontrolsusceptibilitytocontacthypersensitivity:S.F.Martin,etal.;J.Exp.Med.205,2151(2008)
