Because qFlamma® Red Maleimide developed by advanced manufacturing technology of BioActs has a wide range of quench that is 450 ~ 700 nm, It can combine with any fluorescent dyes in Dual labeled probe. In any combination with (in dual labeled probe (Dual labeled probe) it can emit its features. Dual labeled probe is a combination of Fluorescent dye and Quencher based on the principle of Fluorescence resonance energy transfer (FRET). In general, it is labeled on nucleic acid, peptide or nanoparticles and utilized in areas such as qPCR, measurement of enzyme activity. qFlamma® Red Maleimide that features high water solubility bonds specifically to Thiol group (-SH) which is contained in Cysteine of protein. After conducting pre-treatment process that reduces disulfide bond in protein using reducing agent such as DTT, TCEP or 2-mercaptoethanol prior to reaction, it can conjugate qFlamma® Red Maleimide and protein. Excitation (nm): 556nm.