Because qFlamma® Black01 Vinylsulfone developed by advanced manufacturing technology of BioActs has a wide range of quench that is 400 ~ 800 nm, It can combine with any fluorescent dyes in Dual labeled probe. In any combination with (in dual labeled probe (Dual labeled probe) it can emit its features. Dual labeled probe is a combination of Fluorescent dye and Quencher based on the principle of Fluorescence resonance energy transfer (FRET). In general, it is labeled on nucleic acid, peptide or nanoparticles and utilized in areas such as qPCR, measurement of enzyme activity. qFlamma® Black01 Vinylsulfone that features high water solubility reacts to primary amine (-NH2) in very stable way even in aqueous solution depending on the characteristics of Vinylsulfone, which is an independent labeling functional group of BioActs. Therefore, it can label peptide and Amine-modified Oligonucleotide in aqueous solution, and spacer option such as PEGylation provides a wide range of utilization area.