| ProductDetails | |
|---|---|
| Synonyms | 4-Nitro-7-piperazinobenzofurazan |
| ProductType | Chemical |
| Properties | |
| Formula | C10H11N5O3 |
| MW | 249.23 |
| CAS | 139332-66-4 |
| Source/HostChemicals | Synthetic. |
| PurityChemicals | ≥99%(HPLC) |
| Appearance | Redtobrownpowder. |
| Solubility | SolubleinDMSO. |
| Identity | Determinedby1H-NMR. |
| Declaration | ManufacturedbyChemodex. |
| OtherProductData | ClickhereforOriginalManufacturerProductDatasheet |
| InChiKey | MVLWYDGJBGPXOL-UHFFFAOYSA-N |
| ShippingandHandling | |
| Shipping | AMBIENT |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Protectfromlightandmoisture. |
| Use/StABIlity | Stableforatleast2yearsafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet |  DownloadPDF |
Thiscommonfluorescentreagentoftenusedincarbohydrateanalysis,isalsousefulinfluorescencevisualizationoflysosomesinlivecelllinesinculture.ThisfluorophorethathastheunusualenvironmentallysensitivepropertyofincreasingfluorescenceinlowpHenvironments.ComparisonofstainingpatternswiththecommonLysoTracker™orLysoSensor™dyesindicatedcomparablestaining,albeitusingahigherconcentrationofNBD-piperazine(1μMversus100nMfortheMPdyes)butatalowercostperassay.Alsousedforthedeterminationofmono-anddiisocyantesinairsamples,anddeterminationoflactate,hydroxybutyrateandotherintermediatesinbodyfluids.ThepiperazinomoietyreactsreADIlywithisocyantestoformthecorrespondingurea-derivatives.ThederivativescanbedetectedbyLCwithUVorfluorescent-detection.Spectraldata:λex=470nm,λem=540nm.
(1)T.M.Toyo"oka,etal.;J.Chromatogr.588,61(1991)|(2)T.M.Toyo"oka,etal.;Anal.Chim.Acta465,111(2002)|(3)T.Santa,etal.;Biomed.Chromatogr.16,523(2002)|(4)M.Vogeletal.;Anal.Chem.74,6418(2002)|(5)A.Ostinetal.;J.Environ.Monit.5,100(2003)|(6)J.-A.Leeetal.;AnalyticaChimicaActa534(2),185(2005)|(7)K.Ishiguroetal.;BioTechniques45(4),465(2008)
