Adipogen/JC-1/AG-CR1-3568-5001/5 x 1 mg
商品编号：
AG-CR1-3568-5001
品牌：
Adipogen Inc
市场价：
¥4400.00
美元价：
2640.00
产品分类：
其他试剂
公司分类：
Other_reagents
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductDetails | |
|---|---|
| Synonyms | 5,5",6,6"-Tetrachloro-1,1",3,3"-tetraethylbenzimidazolylcarbocyanineiodide |
| ProductType | Chemical |
| Properties | |
| Formula | C25H27Cl4IN4 |
| MW | 652.2 |
| CAS | 3520-43-2and47729-63-5 |
| PurityChemicals | ≥98%(NMR) |
| Appearance | Redpowder. |
| Solubility | SolubleinDMSO,ethanol,methanoloracetone. |
| Identity | Determinedby1H-NMRand13C-NMR. |
| OtherProductData | ApplicationProtocols:Seeliteratures8,12or13orthewebsiteprotocolfromPurdueUniversity. |
| InChiKey | FYNNIUVBDKICAX-UHFFFAOYSA-M |
| ShippingandHandling | |
| Shipping | AMBIENT |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Keepcoolanddry. Protectfromlight. |
| Use/StABIlity | Stableforatleast2yearsafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Themembrane-permeantdual-emissionpotential-sensitiveJC-1dyeiswidelyusedinapoptosisstudiestomonitormitochondrialhealthbyflowcytometry,fluorescencemicroscopyandinmicroplate-basedfluorescentassays.JC-1dyecanbeusedasanindicatorofmitochondrialmembranepotentialinavarietyofcelltypes,includingmyocytesandneurons,aswellasinintacttissuesandisolatedmitochondria.JC-1accumulatesinmitochondria,selectivelygeneratinganorangeJ-aggregateemissionprofile(590nm)inhealthycells.Aftercellinjury,asmembranepotentialdecreases,JC-1monomersaregenerated,resultinginashifttogreenemission(529nm).TheprincipaladvantageofJC-1relativetoothercommonlyemployedfluorescentprobesofmitochondrialmembranepotentialisthatitallowsqualitativevisualization,consideringtheshiftfromorangetogreenfluorescenceemission,andquantitativedetection,consideringthefluorescenceintensityratio.
ProductReferences
- J-aggregateformationofacarbocyanineasaquantitativefluorescentindicatorofmembranepotential:M.Reers,etal.;Biochemistry30,4480(1991)
- IntracellularheterogeneityinmitochondrialmembranepotentialsrevealedbyaJ-aggregate-forminglipophiliccationJC-1:S.T.Smiley,etal.;PNAS88,3671(1991)
- AnewmethodforthecytofluorimetricanalysisofmitochondrialmembranepotentialusingtheJ-aggregateforminglipophiliccation5,5",6,6"-tetrachloro-1,1",3,3"-tetraethylbenzimidazolcarbocyanineiodide(JC-1):A.Cossarizza,etal.;BBRC197,40(1993)
- MitochondrialmembranepotentialmonitoredbyJC-1dye:M.Reers,etal.;MethodsEnzymol.260,406(1995)
- JC-1,butnotDiOC6(3)orrhodamine123,isareliablefluorescentprobetoassessdeltapsichangesinintactcells:implicationsforstudiesonmitochondrialfunctionalityduringapoptosis:S.Salvioli,etal.;FEBSLett.411,77(1997)
- FunctionalassayofmultidrugresistantcellsusingJC-1,acarbocyaninefluorescentprobe:J.M.Kühnel,etal.;Leukemia11,1147(1997)
- Flowcytometricmeasurementofmitochondrialmassandfunction:anovelmethodforassessingchemoresistance:M.Mancini,etal.;Ann.Surg.Oncol.5,287(1998)
- FlowcytometricanalysisofmitochondrialmembranepotentialusingJC-1:A.Cossarizza&S.Salvioli;Curr.Protoc.Cytom.Chapter9,Unit9.14(2001)(Protocol)
- JC-1:averysensitivefluorescentprobetotestPgpactivityinadultacutemyeloidleukemia:O.Legrand,etal.;Blood97,502(2001)
- MitochondrialandnonmitochondrialreductionofMTT:interactionofMTTwithTMRE,JC-1,andNAOmitochondrialfluorescentprobes:T.Bernas&J.Dobrucki;Cytometry47,236(2002)
- JC-1,asensitiveprobeforasimultaneousdetectionofP-glycoproteinactivityandapoptosisinleukemiccells:D.Chaoui,etal.;CytometryBClin.Cytom.70,189(2006)
- PolychromaticanalysisofmitochondrialmembranepotentialusingJC-1:E.Lugli,etal.;Curr.Protoc.Cytom.Chapter7,Unit7.32(2007)(Protocol)
- LabelingmitochondriawithJC-1:B.Chazotte;ColdSpringHarb.Protoc.2011,(2011)(Protocol)
- StableOxidativeCytosineModificationsAccumulateinCardiacMesenchymalCellsfromType2DiabetesPatients:RescuebyAlpha-KetoglutarateandTET-TDGFunctionalReactivation:F.Spallotta,etal.;Circ.Res.Aheadofprint(2017)
品牌介绍
Adipogen公司由三个子公司组成，分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国，运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发，如癌症、免疫、炎症、代谢疾病（糖尿病、肥胖）、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外，也可提供抗体和蛋白产品，Adipogen拥有自己的化学实验室，致力于开发创新型小分子化合物。
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