| ProductDetails | |
|---|---|
| ProductType | Set |
| Properties | |
| ApplicationSet | CompoundScreening |
| KitContains | iODN2088(ClassI)Endotoxin-free(sterile),100μg(Cat.No.:IAX-200-050-C100) |
| OtherProductData | Endotoxinfree:<0.002EU/μg |
| Declaration | ManufacturedbyInnaxon. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | +4°C |
| HandlingAdvice | Avoidfreeze/thawcycles. Afterreconstitution,preparealiquotsandkeepaqueousstocksolutionsfor1dayat4°Corstoreat-20°C(shelf-life6months). |
| Use/StABIlity | Stableforatleast2yearsafterreceiptwhenstoredat+4°C. |
| Documents | |
| Manual | No |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Inrecentyearsseveralgroupshavestudiedthesequencerequirements,specificity,signallingpathwaysandkineticsoftheTLR(Toll-likereceptor)9suppressionbyinhibitoryoligonucleotidemotifs,whichledtoaclassofnovelinhibitoryoligonucleotide(iODNs),thatisindependentofthepreviouslythoughtspeciespreference.SubsequentlyithasbeendiscoveredthattelomericDNArepeats(TTAGGG)ncanblockimmuneactivationbyCpG-ODNs.Short,11-15baselongoligonucleotidesweresynthesizedthatwerecapableofpotentlyinhibitingCpG-stimulation.TheoptimalinhibitoryDNAmotifconsistsofapyrimidine-richtriplet,preferablyCCT,whichispositioned5-totheGGGsequenceinasinglestrandedDNAmolecule.Additionally,boththeoptimalspacingbetweentheCCTandGGGmotifs,aswellastheirrelativeordertoeachother,isofcrucialimportancefortheinhibitoryDNAaction.Interestingly,althoughbothTLR7/TLR8ligandsandbacterialDNAsharetheendosomalcompartmentforreceptorbindingandsignaltransduction,certainiODNs(G-type)suppressonlyTLR9-mediatedactivation,whereasprototypeclassIiODNmayalsointerferewiththeactivationviatheTLR7/TLR8pathway.Recently,intriguingevidencehasbeenpresentedthatforsomeiODNclassestheimmuno-modulatoryBIOLOGicalactivityshowsonlylimitedsequencedependencyormaynoteveninvolveTLR-mediateduptakeandsignalingpathways.ForexampleiODNsoftheclassIIarethoughttoactonimmuneactivationthroughinhibitionofSTATsignallingandindependentofTLRsignallingviabindingtoayettobeidentified"ODN-receptor".SlightlymodifiedphosphodiesterversionsofthemostpotentinhibitoryODNswerealsoabletoprofoundlyblocktheimmuneactivationofmacrophagesandjustrecentlyprovetobevaluabletoolsforinvivouseinexperimentalanimalmodelsofinflammatoryandauto-immunediseases.BasedupontheserecentinsightsthefollowingclassificationforiODNshasbeensuggested:
ClassI:G-stretchODNs:TLR9-specificcompetitors
ClassII:ODNswithtelomericrepeats:TLR-independentinhibitorsofSTATsignalling
ClassIII:InhibitorsofDNAuptakeinasequenceindependentmanner
ClassIV:LongphosphorothioateODNsasdirectcompetitorsofTLR9signallinginasequenceindependentmanner
- DNAMotifssuppressingTLR9responses:A.Trieu,etal.;Crit.Rev.Immunol.26,527(2006)
- Inhibitoryoligodeoxynucleotides-therapeuticpromiseforsystemicautoimmunediseases?P.Lenert;Clin.Exp.Immunol.140,1(2005)
- Immunotherapeuticutilityofstimulatoryandsuppressiveoligodeoxynucleotides:K.J.Ishii,etal.;Curr.Opin.Mol.Ther.6,166(2004)
- Suppressiveoligodeoxynucleotidesprotectmicefromlethalendotoxicshock:H.Shirota,etal.;J.Immunol.174,4579(2005)
- Toll-likereceptor9inhibitionreducesmortalityinpolymicrobialsepsis:G.Plitas,etal.;J.Exp.Med.205,1277(2008)
