| ProductDetails | |
|---|---|
| Synonyms | FDA;3,6-Diacetoxyfluoran;Di-O-Acetylfluorescein;Cellstain-FDA;NSC4726;NSC667259 |
| ProductType | Chemical |
| Properties | |
| Formula | C24H16O7 |
| MW | 416.38 |
| CAS | 596-09-8 |
| Source/HostChemicals | Synthetic. |
| PurityChemicals | ≥95%(NMR) |
| Appearance | Whitetooff-whitepowder. |
| Solubility | SolubleinDMSO,ethanol,water,chloroformoracetone(25mg/ml). |
| Identity | Determinedby1H-NMR. |
| Declaration | ManufacturedbyChemodex. |
| OtherProductData | ClickhereforOriginalManufacturerProductDatasheet Ourproductdescriptionmaydifferslightlyfromtheoriginalmanufacturersproductdatasheet. |
| InChiKey | CHADEQDQBURGHL-UHFFFAOYSA-N |
| ShippingandHandling | |
| Shipping | AMBIENT |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Keepcoolanddry. Protectfromlightandmoisture. |
| Use/StABIlity | Stableforatleast2yearsafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet |  DownloadPDF |
FDAisanon-fluorescenthydrophobicfluoresceinderivativethatcanpassthroughthecellmembranewhereuponintracellularesteraseshydrolyzethediacetategroupproducingthehighlyfluorescentproductfluorescein.ThefluoresceinmoleculesaccumulateincellsthatpossessintactmembranessothegreenfluorescencecanbeusedasaMarkerofcellviability.Cellsthatdonotpossessanintactcellmembraneoranactivemetabolismmaynotaccumulatethefluorescentproductandthereforedonotexhibitgreenfluorescence.FDAmaybeusedincombinationwithPIstainingasthenon-viablecellstakeupthePIandstaindeadcellsredwhereasviablecellsdonottakeupthePIandshouldonlystaingreen.This2-colorseparationofnon-viableandviablecellsmayprovideamoreaccuratequantitationofcellviabilitythansinglecoloranalysis.FDAisusedinstudiesonintracellularinteractionsandmembranepermeability,forflowcytometryandasesterasesubstrate.Spectraldata:&lamBDa;ex=494nm,λem=521nm.
(1)B.Rotman,etal.;PNAS55,134(1966)|(2)H.R.Hulett,etal.;Science166,747(1969)|(3)K.H.Jones,etal.;J.Histochem.Cytochem.33,77(1985)|(4)K.McGinnes,etal.;J.Immunol.Methods86,7(1986)|(5)D.D.Ross,etal.;CancerRes.49,3776(1989)|(6)E.Prosperi;Histochem.J.22,227(1990)|(7)M.Miyamoto,etal.;CellTransplant9,681(2000)|(8)S.Wanandy,etal.;J.Microbiol.Methods60,21(2005)|(9)J.Wang,etal.;DrugMetab.Dispos.39,1329(2011)|(10)N.Saruyama,etal.;Anal.Biochem.441,58(2013)
