Adipogen/DAPI . dihydrochloride/AG-CR1-3668-M005/5 mg
商品编号：
AG-CR1-3668-M005
品牌：
Adipogen Inc
市场价：
¥700.00
美元价：
420.00
产品分类：
其他试剂
公司分类：
Other_reagents
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductDetails | |
|---|---|
| Synonyms | 4",6-Diamidino-2-phenylindoledihydrochloride;2-(4-Amidinophenyl)-6-indolecarbamidinedihydrochloride;FxCycleViolet |
| ProductType | Chemical |
| Properties | |
| Formula | C16H15N5.2HCl |
| MW | 277.3.72.9 |
| CAS | 28718-90-3 |
| RTECS | NL5995050 |
| PurityChemicals | ≥97%(HPLC) |
| Appearance | Yellowsolid. |
| Solubility | Solubleinwater(10mg/ml).Mayrequireheatingandsonication.SlightlysolubleinDMSO,ethanol,methanolorPBS. |
| Identity | Determinedby1H-NMR. |
| OtherProductData | Note:Stocksolutions(1mg/ml)shouldbestoredinthedarkandarestableinthedarkat2-8°Cfor1-2weeks.Forlong-termstorage,thesolutionscanbedividedintoaliquotsandstoredat-20°C. |
| InChiKey | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
| ShippingandHandling | |
| Shipping | AMBIENT |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Keepcoolanddry. Protectfromlightandmoisture. |
| Use/StABIlity | Stableforatleast2yearsafterreceiptwhenstoredat-20°C. Storesolutionsat-20°Cinthedark. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
- CellpermeablefluorescentDNAstain.AT-sequence-specificDNAintercalator.
- Bindstotheminorgrooveofdouble-strandedDNA(preferentiallytoadenineandthymine(AT)richDNA),likeHoechstdyes,formingastablecomplexwhichfluorescesapproximately20timesgreaterthanDAPIalone.SpectralData:Excitation:&lamBDa;ex340nm;Emission:λem488nm(onlyDAPI).Excitation:λex360nm;Emission:λem460nm(DAPI-DNAcomplex).
- CommonlyusedtostainDNAandchromosomesforfluorescentmicroscopyandflowcytometryapplications.
- DAPIisoftenusedasacounterstain,asitsultravioletexcitation(λex360nm)andblueemission(λem460nm)wavelengthsseparateitnicelyfrommanypopularprimaryfluorophores.
- Canbeusedoneitherfixedorlivecells,althoughitpassesthroughthemembranelessefficientlyinlivecellsandthereforetheeffectivenessofthestainisloweranddemandshigherconcentrationstobeused.
- ReversIBLeinhibitorofS-adenosyl-L-methioninedecarboxylaseandKAO(diamineoxidase).
ProductReferences
- AsimplecytochemicaltechniquefordemonstrationofDNAincellsinfectedwithmycoplasmasandviruses: W.C.Russell,etal.;Nature253,461(1975)
- DIPIandDAPI:fluorescencebandingwithonlynegligliblefADIng:W.Schnedl,etal.;Hum.Genet.36,167(1977)
- AnevaluationofDNAFluorochromes,stainingtechniques,andanalysisforflowcytometry.I.Unperturbedcellpopulations:I.W.Taylor&B.K.Milthorpe;J.Histochem.Cytochem.28,1224(1980)
- AcomparativestudyofDAPI,DIPI,andHoechst33258and33342aschromosomalDNAstains:F.Otto&K.C.Tsou;StainTechnol.60,7(1985)
- EstimationoftotalDNAincrudeextractsofplantleaftissueusing4",6-diamidino-2-phenylindole(DAPI)fluorometry:L.S.Lee&H.M.Garnett;J.Biochem.Biophys.Methods26,249(1993)
- 4",6-Diamidino2-phenylindoleisanewreversibleinhibitorofdiamineoxidaseandS-adenosyl-L-methioninedecarboxylasefrommammaliantissues:C.Cubria,etal.;Comp.Biochem.Physiol.C.105,251(1994)
- Flowcytometricdetectionofapoptosis:comparisonoftheassaysofinsituDNAdegradationandchromatinchanges:M.A.Hotz,etal.;Cytometry15,237(1994)
- DAPI:aDNA-specificfluorescentprobe:J.Kapuscinski;Biotech.Histochem.70,220(1995)
- TheuseofDAPIasareplacementforethidiumbromideinforensicDNAanalysis:E.Buel&M.Schwartz;J.ForensicSci.40,275(1995)
- Visualizingchromatinandchromosomesinlivingcells:D. Zink,etal.;Methods:ACompaniontoMethodsinEnzymology29,42(2003)
- PropidiumIodide(PI)orDAPIStainingofUnfixedSolidTissuesforFlowCytometry:L.Rodgers;CSHProtoc.2006,pdb.prot4438(2006)
- DAPIStainingofDrosophilaEmbryos:W.F.Rothwell&W.Sullivan;CSHProtoc.2007,pdb.prot4834(2007)
- LabelingnuclearDNAusingDAPI:B.Chazotte;CSHProtoc.2011,pdb.prot5556(2011)
- AninvestigationofthephotophysicalpropertiesofminorgrooveboundandintercalatedDAPIthroughquantum-mechanicalandspectroscopictools:A.Biancardi,etal.;Phys.Chem.Chem.Phys.15,4596(2013)
- DAPIstainingandfluorescencemicroscopytechniquesforphytoplasmas:N.M.Andrade&N.L.Arismendi;MethodsMol.Biol.938,115(2013)
- ThehazardsofDAPIphotoconversion:effectsofdye,mountingmediaandfixative,andhowtominimizetheproblem:M.Jez,etal.;Histochem.CellBiol.139,195(2013)
- FluorescencelifetimeimagingofDAPI-stainednucleiasanoveldiagnostictoolforthedetectionandclassificationofB-cellchroniclymphocyticleukemia:G.Yahav,etal.;CytometryA89,644(2016)
品牌介绍
Adipogen公司由三个子公司组成，分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国，运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发，如癌症、免疫、炎症、代谢疾病（糖尿病、肥胖）、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外，也可提供抗体和蛋白产品，Adipogen拥有自己的化学实验室，致力于开发创新型小分子化合物。
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