| Product Details | |
|---|---|
| Synonyms | Fluorescein 6-isothiocyanate; Fluorescein-4-isothiocyanate; 2-(3-Hydroxy-6-oxoxanthen-9-yl)-4-isothiocyanatobenzoic acid; FITC Isomer II; FITC-II |
| Product Type | Chemical |
| Properties | |
| Formula | C21H11NO5S |
| MW | 389.39 |
| CAS | 18861-78-4 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥90% (HPLC) |
| Appearance | Yellow to brown powder. |
| Solubility | Soluble in ethanol, DMSO or DMF. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet. |
| InChi Key | GTQFZXYECNSNNC-UHFFFAOYSA-N |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Keep cool and dry. Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | No |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
6-Fluorescein isothiocyanate (6-FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. Spectral data: λex=492nm, λem=518nm.
(1) T.J. Porro, et al.; Stain Technol. 38, 37 (1963) | (2) H.S. Corey & R.M. McKinney; Anal. Biochem. 10, 387 (1965) | (3) R.M. McKinney, et al.; Anal. Biochem. 29, 526 (1969) | (4) J. Sinsheimer, et al.; Anal. Biochem. 57, 227 (1974) | (5) D.L. Reynolds & L.A. Pachla; J. Pharm. Sci. 74, 1091 (1985) | (6) R. Zuckermann, et al.; Nucl. Acids Res. 15, 5305 (1987) | (7) C.A. Swindlehurst & E.W. Voss; Biophys. J. 59, 619 (1991) | (8) C. Wang, et al.; BBRC 331, 351 (2005)
