| ProductDetails | |
|---|---|
| Synonyms | 5-CDCFDA;5-Carboxy-2",7"-dichlorofluoresceindiacetate;5-Carboxy-2",7"-dichlorofluoresceinDA |
| ProductType | Chemical |
| Properties | |
| Formula | C25H14Cl2O9 |
| MW | 529.28 |
| CAS | 144489-09-8 |
| Source/HostChemicals | Synthetic. |
| PurityChemicals | ≥95%(NMR) |
| Appearance | Off-whitepowder. |
| Solubility | SolubleinDMSO,DMF,acetonitrile,methanolorchloroform. |
| Identity | Determinedby1H-NMR. |
| Declaration | ManufacturedbyChemodex. |
| OtherProductData | ClickhereforOriginalManufacturerProductDatasheet Ourproductdescriptionmaydifferslightlyfromtheoriginalmanufacturersproductdatasheet. |
| InChiKey | AJIVVDSJIOOZOJ-UHFFFAOYSA-N |
| ShippingandHandling | |
| Shipping | AMBIENT |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Keepcoolanddry. Protectfromlightandmoisture. |
| Use/StABIlity | Stableforatleast2yearsafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet |  DownloadPDF |
Non-activated,amine-reactivefluorescentprobe.Single5-isomerofCDCFDAthatcanbeusedinbiomoleculesconjugation.CDCFDAisanamine–reactivefluoresceindiacetate(FDA)derivativeusedtoprepareavarietyofFDAconjugates.CDCFDAisanon-fluorescentmoleculethatdiffuseintocellsandishydrolyzedbyintracellularnon-specificesterases,yieldingafluorescentprobe.Theprobeaccumulatesexclusivelyinviablecellswithintactcellmembranes.CFCFDAdoesnotstaindeadcells.CDCFDAlabelingcanbeusedformonitoringcellsbyflowcytometryorfluorescencemicroscopy.Theprobeisalsofrequentlyusedtomonitorreactiveoxygenspecies(ROS)generationinlivecellsandintracellularpHlevels.Spectraldata:&lamBDa;ex=504nm,λem=529nm.
(1)C.K.Raymondetal.;J.CellBiol.111,877(1990)|(2)J.P.Kehrer&T.Paraidathathu;FreeRad.Res.Commun.16,217(1992)|(3)Q.P.Lloyd,etal.;J.Biol.Chem.270,22445(1995)|(4)A.L.Inselman,etal.;Curr.Microbiol.38,295(1999)|(5)M.J.Zamek-Gliszczynski,etal.;J.Pharmacol.Exp.Therap.304,801(2003)|(6)L.Li,etal.;FreeRADIc.Res.42,354(2008)|(7)M.Medhora,etal.;Am.J.Physiol.LungCellMol.Physiol.294,L902(2008)|(8)S.Siissalo,etal.;Eur.J.Pharm.Biopharm.71,332(2009)|(9)A.I.Pogue,etal.;Int.J.Mol.Sci.13,9615(2012)|(10)J.Roh,etal.;RedoxBiol.11,254(2016)|(11)J.L.Roh,etal.;CancerLett.381,96(2016)
