| Product Details | |
|---|---|
| Synonyms | 6-(Fluorescein-5(6)-carboxamido)hexanoicacid N-succinimidyl ester; 5(6)-FAM-X, SE; C6-F-NHS; FHS |
| Product Type | Chemical |
| Properties | |
| Formula |
C31H26N2O10 |
| MW | 586.6 |
| CAS | 114616-31-8 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥75% (HPLC) |
| Appearance | Yellow to orange powder. |
| Solubility | Soluble in dimethylformamide or methanol. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | AZTAZTWVNFVDPK-UHFFFAOYSA-N |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice |
Keep cool and dry. Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | No |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Amine-reactive fluorescent probe for introducing a fluorescein label at a nucleophilic residue of a target molecule. This fluorescein labeling reagent has an added (X) spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. The reactive N-hydroxysuccinimide (NHS) group can be used for easy attachment to proteins or amine containing nucleotides using simple procedures. Spectral properties: λex 490 nm; λem 514 nm in DMF (pH 8.2) and λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0.
(1) G.P. Jamieson, et al.; Cytometry 14, 32 (1993) | (2) K.A. Cruickshank; Anal. Biochem. 269, 21 (1999) | (3) J.J. MacLean, et al.; Biophys. J. 78, 1441 (2000) | (4) W.C. Cooper, et al.; Biophys. J. 78, 1449 (2000) | (5) M.P. Gygi, et al.; Nucleic Acids Res. 30, 2790 (2002)
