| ProductDetails | |
|---|---|
| ProductType | Kit |
| Properties | |
| ApplicationSet | QuantitativeELISA |
| Specificity | SpecifictoFe2+andFe3+. |
| Crossreactivity | All |
| Quantity | Enoughreagentsfor100tests. |
| Range | 10-1"000μg/dL |
| SampleType | CellLysate Plasma Saliva Serum TissueSupernatant Urine |
| DetectionType | Colorimetric |
| KitContains | 1x16ml R-1 Buffer(readytouse) 1x7ml R-2 Buffer(readytouse) 1x0.7ml R-3 Chelatecolor(readytouse) 1x1.5ml STD Iron(Fe)standard200µg/dL(readytouse) |
| OtherProductData | ThisMC-ReagentIronAssayKitisadirectcolorimetricassaybasedontheNitroso-PSAPmethodwithoutdeproteinizationofthesample.Aweakacidbufferdissociatesironfromthetransferrin-ironcomplexandisreducedbyareductant(Ferric>>Ferrous).FerrousionsformacomplexwiththechromogenNitroso-PSAP.Thecolorintensityisproportionaltotheironconcentrationinthesample.AbsorbanceoftheFe2+-complexismeasuredat750nm.Wavelengthrangeofsensitivity:740~760nm. FeaturesofthisAssay:Quick&Easytouse•Speciesindependent•Highlysensitive,stableandsuitableforhigh-throughputtesting•Notoxicsubstances•Forcelllysates,serum,plasmaandwidevarietyofBIOLOGicalsamples |
| Declaration | ManufacturedbyJaICA. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | +4°C |
| HandlingAdvice | Donotfreeze. |
| Use/StABIlity | 12monthsafterthedayofmanufacturing.SeeexpirydateonELISAKitbox. |
| Documents | |
| Manual |  DownloadPDF |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Ironisamineral(functioningasanenzymecofactor)thatplaysanessentialroleinmanybiologicalprocesses.Itisessentialtonearlyallknownorganisms.Asatransitionelementitcanformarangeofoxidationstates,themostcommonbeingFe2+(orferrousiron)andFe3+(orferriciron).IngestedironismainlyabsorbedintheformofFe2+.Thetrivalentformandtheheme-boundFe2+-componentofironisreducedbyvitaminC.Beforepassingintotheplasma,itisoxidizedbyceruloplasmintoFe3+andboundtotransferrintoformatransferrin-ironcomplex.Ironisgenerallystoredinthecentreofmetalloproteins,inthehemecomplex,andinoxygencarrierproteins.Iron-containingproteinsparticipateinmanyreactions,oftenutilizingtransitorychangesintheoxidationstateofirontocarryoutchemicalreactions.Ironisimportantforredoxreactions,oxygentransport(e.g.hemoglobin),short-termoxygenstorage(e.g.myoglobin)andenergygeneration.Irondeficiencyhasmanyadverseconsequences,includinganemia,hemochromatosis,chronicrenaldiseaseandinchildren,behavioralandlearningdisorders.Ironexcessistoxictothebody,harmingtheheart,liver,skin,pancreaticisletbetacells,bones,joints,andpituitarygland.Maintainingproperironbalanceisessentialformaintaininghomeostasisandhealth.
- Anautomatedmethodformeasurementofserumironandunsatur-atedironbindingcapacityusingnitroso-PSAP:M.Yamada,etal.;Jpn.J.Lab.Auto.13,p659(1988)
- FecA1,abacterialirontransporter,determinesthesurvivalofHelicobacterpyloriinthestomach:H.Tsugawa,etal.;FreeRADIc.Biol.Med.52,1003(2012)
- EstrogenRegulatesHepcidinExpressionviaGPR30-BMP6-DependentSignalinginHepatocytes:Y.Ikeda,etal.;PLoSOne7,e40465(2012)
- GRK6deficiencyinmicecausesautoimmunediseaseduetoimpairedapoptoticcellclearance;M.Nakaya;Nat.Comm.4,1532(2013)
- Superparamagneticnanoparticleclustersforcancertheranosticscombiningmagneticresonanceimagingandhyperthermiatreatment:K.Hayashi,etal.;Theranostics3,366(2013)
