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Adipogen/Iron Colorimetric Assay Kit (Ferrozine Method)/JAI-CFE-005/100 tests
| ProductDetails | |
|---|---|
| ProductType | Kit |
| Properties | |
| ApplicationSet | QuantitativeELISA |
| Specificity | SpecifictoFe2+andFe3+. |
| Crossreactivity | All |
| Quantity | Enoughreagentsfor100tests. |
| Range | 5-1"000μg/dL |
| SampleType | CellLysate Plasma Saliva Serum TissueSupernatant Urine |
| DetectionType | Colorimetric |
| KitContains | 1x20ml R-1 Buffer(readytouse) 1x0.8ml R-2 Chelatecolor(readytouse) 1x4ml STD Ironstandard200µg/dL(readytouse) |
| OtherProductData | ThisMC-ReagentIronAssayKitisadirectcolorimetricassaybasedontheFerroZine®methodwithoutdeproteinizationofthesample.Aweakacidbufferdissociatesironfromthetransferrin-ironcomplexandisreducedbyareductant(Ferric>>Ferrous).FerrousionsformacomplexwiththechromogenFerrozine.Thecolorintensityisproportionaltotheironconcentrationinthesample.AbsorbanceoftheFe2+-complexismeasuredat560nm.Wavelengthrangeofsensitivity:540~580nm. FeaturesofthisAssay:Quick&Easytouse•Speciesindependent•Highlysensitive,stableandsuitableforhigh-throughputtesting•Notoxicsubstances•Forcelllysates,serum,plasmaandwidevarietyofBIOLOGicalsamples |
| Declaration | ManufacturedbyJaICA. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | +4°C |
| HandlingAdvice | Donotfreeze. |
| Use/StABIlity | 12monthsafterthedayofmanufacturing.SeeexpirydateonELISAKitbox. |
| Documents | |
| Manual |  DownloadPDF |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Ironisamineral(functioningasanenzymecofactor)thatplaysanessentialroleinmanybiologicalprocesses.Itisessentialtonearlyallknownorganisms.Asatransitionelementitcanformarangeofoxidationstates,themostcommonbeingFe2+(orferrousiron)andFe3+(orferriciron).IngestedironismainlyabsorbedintheformofFe2+.Thetrivalentformandtheheme-boundFe2+-componentofironisreducedbyvitaminC.Beforepassingintotheplasma,itisoxidizedbyceruloplasmintoFe3+andboundtotransferrintoformatransferrin-ironcomplex.Ironisgenerallystoredinthecentreofmetalloproteins,inthehemecomplex,andinoxygencarrierproteins.Iron-containingproteinsparticipateinmanyreactions,oftenutilizingtransitorychangesintheoxidationstateofirontocarryoutchemicalreactions.Ironisimportantforredoxreactions,oxygentransport(e.g.hemoglobin),short-termoxygenstorage(e.g.myoglobin)andenergygeneration.Irondeficiencyhasmanyadverseconsequences,includinganemia,hemochromatosis,chronicrenaldiseaseandinchildren,behavioralandlearningdisorders.Ironexcessistoxictothebody,harmingtheheart,liver,skin,pancreaticisletbetacells,bones,joints,andpituitarygland.Maintainingproperironbalanceisessentialformaintaininghomeostasisandhealth.
- FecA1,abacterialirontransporter,determinesthesurvivalofHelicobacterpyloriinthestomach:H.Tsugawa,etal.;FreeRADIc.Biol.Med.52,1003(2012)
- EstrogenRegulatesHepcidinExpressionviaGPR30-BMP6-DependentSignalinginHepatocytes:Y.Ikeda,etal.;PLoSOne7,e40465(2012)
- GRK6deficiencyinmicecausesautoimmunediseaseduetoimpairedapoptoticcellclearance;M.Nakaya;Nat.Comm.4,1532(2013)
- Dietaryironrestrictioninhibitsprogressionofdiabeticnephropathyindb/dbmice:Y.Ikeda;Am.J.Physiol.Renal.Physiol.304,F1028(2013)
- IronChelationbyDeferoxaminePreventsRenalInterstitialFibrosisinMicewithUnilateralUreteralObstruction:Y.Ikeda;PLoSOne9,e89355(2014)
