| ProductDetails | |
|---|---|
| ProductType | Kit |
| Properties | |
| ApplicationSet | QuantitativeELISA |
| Specificity | SpecifictoCu2+andCu+. |
| Crossreactivity | All |
| Quantity | Enoughreagentsfor100tests. |
| Range | 3-400μg/dL |
| SampleType | CellLysate Plasma Saliva Serum TissueSupernatant Urine |
| DetectionType | Colorimetric |
| KitContains | 1x24ml R-1 Buffer(withreductant)(readytouse) 1x0.5ml R-2 Chelatecolor(readytouse) 1x1.2ml STD Copper(Cu)standard200µg/dL(readytouse) |
| OtherProductData | ThisMC-ReagentCopperAssayKitisadirectcolorimetricassaybasedonthe3,5-DiBr-PAESAmethodwithoutdeproteinizationofthesample.Aweekacidbufferdissociatescopperfromtheceruloplasmin-coppercomplexandisreducedbyascorbicacid(Cu2+>>Cu+).Cu+ionsformabluecoloredcomplexwiththechromogen3,5-DiBr-PAESA.Thecolorintensityisproportionaltothecopperconcentrationinthesample.AbsorbanceoftheCu+-complexismeasuredat580nm.Wavelengthrangeofsensitivity:570~590nm. FeaturesofthisAssay:Quick&Easytouse•Speciesindependent•Highlysensitive,stableandsuitableforhigh-throughputtesting•Notoxicsubstances•Forcelllysates,serum,plasmaandwidevarietyofBIOLOGicalsamples |
| Declaration | ManufacturedbyJaICA. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | +4°C |
| HandlingAdvice | Donotfreeze. |
| Use/StABIlity | 12monthsafterthedayofmanufacturing.SeeexpirydateonELISAKitbox. |
| Documents | |
| Manual |  DownloadPDF |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Copper(Cu+)isanessentialtraceelement(i.e.,micronutrient)andcofactor.95%ofcopperinplasmaisincomplexwithenzymes,mostlywithceruloplasmin,severaloxidasesanddismutases,whichplayimportantrolesinironandcatecholaminemetabolism,freerADIcalscavenging,andinthesynthesisofhaemoglobinandelastin.Themicronutrientisnecessaryforthepropergrowth,development,andmaintenanceofbone,connectivetissue,brain,heart,andmanyotherbodyorgans.Copperisinvolvedintheformationofredbloodcells,theabsorptionandutilizationofiron,themetabolismofcholesterolandglucose,andthesynthesisandreleaseoflife-sustainingproteinsandenzymes.Theseenzymesinturnproducecellularenergyandregulatenervetransmission,bloodclotting,andoxygentransport.Copperstimulatestheimmunesystemtofightinfections,torepairinjuredtissues,andtopromotehealing.Copperalsohelpstoneutralize"free-radicals",whichcancauseseveredamagetocells.Copperdeficiencylowerstheanti-oxidantfunctioninvivoandhasbeenassociatedwithmentalretardation,depigmentation,anemia,hypotoniaandscorbuticchangesinbone.Levelsofcopperarekeydiagnosticindicatorofdiseasessuchascardiopathy,osteoporosis,osteoarthritis,MenkessyndromeandWilson"sdisease.Acutecoppertoxicitycancausegastroenteritisandacuterenalfailure.
- Enhancedbiofilmformationand/orcellviabilitybypolyaminesthroughstimulationofresponseregulatorsUvrYandCpxRinthetwo-componentsignaltransducingsystems,andribosomerecyclingfactor:A.Sakamoto,etal.;Int.J.Biochem.CellBiol.44,1877(2012)
- APrimaryRoleforDisulfideFormationintheProductiveFoldingofProkaryoticCu,Zn-superoxidedismutase:Y.Sakurai,etal.;J.Biol.Chem.289,20139(2014)
