Adipogen/anti-VASP, mAb (IE273)/AG-20T-0002-C050/50 µg
商品编号：
AG-20T-0002-C050
品牌：
Adipogen Inc
市场价：
¥7000.00
美元价：
4200.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductDetails | |
|---|---|
| Synonyms | Vasodilator-stimulatedPhosphoprotein |
| ProductType | MonoclonalAntibody |
| Properties | |
| Clone | IE273 |
| Isotype | MouseIgG1 |
| Immunogen/Antigen | PurifiedhumanVASP. |
| Application | ELISA Immunocytochemistry:(1-5μg/ml,1:100-1:500) Immunohistochemistry Immunoprecipitation WesternBlot:(0.01-0.25μg/ml,1:2000-1:50"000) Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| Crossreactivity | Bovine Human Pig Rabbit Sheep |
| Specificity | Recognizesboththe46kDa(Ser157dephospho-)and50kDa(Ser157phospho-)formofhuman,porcine,bovine,sheepandrabbitVASP. |
| Purity | ProteinA/Gpurified. |
| Concentration | 500μg/ml |
| Formulation | Liquid.InPBScontaining2mg/mlBSAand0.02%sodiumazide. |
| OtherProductData | ContainsPositiveControl:Humanplateletprotein(500µg)(Prod.No.AG-35T-0001),suppliedat5mg/mlinSDS-stopsolution(100mMNaCl,73mMTris/HClpH6.7,10mMDTT,8mMEDTA,5%[v/v]glycerol,2%[w/v]SDS,10μg/mlbromophenolbluesodiumsalt).Use5μl(25μg)perlane. |
| AccessionNumber | UniProtIDP50552:VASP |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Avoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
VASP(vasodilator-stimulatedphosphoprotein)isaproline-richproteinsubstrateofcAMP-andcGMP-dependentproteinkinases.VASPisanactin-associatedproteininvolvedinarangeofprocessesdependentonCytoskeletonremodelingandcellpolaritysuchasaxonguidance,lamellipodialandfilopodialdynamics,plateletactivationandcellmigration.VASPpromotesactinfilamentelongation.Itprotectsthebarbedendofgrowingactinfilamentsagainstcappingandincreasestherateofactinpolymerizationinthepresenceofcappingproteins.VASPstimulatesactinfilamentelongationbypromotingthetransferofprofilin-boundactinmonomersontothebarbedendofgrowingactinfilamentsanditplaysaroleinactin-basedmobilityofListeriamonocytogenesinhostcells.Itregulatesactindynamicsinplateletsandplaysanimportantroleinregulatingplateletaggregation.VASPphosphorylationisusedtomonitortheeffectofso-calledantiplateletdrugsthatreduceplateletreactivityandareusedtopreventstentthrombosis,strokesandheartattacksinpatients.PhosphorylationofVASPatSer157causesamobilityshiftinSDSgelelectrophoresisfrom46to50kDa,whichhasbeenusedasaconvenientMarkertomonitorcyclicnucleotide-dependentproteinkinaseactivity.
ProductReferences
- ThemammalianprofilinisoformsdisplaycomplementaryaffinitiesforPIP2andproline-richsequences:A.Lambrechts,etal.;EMBOJ.16,484(1997)
- Anovelproline-richmotifpresentinActAofListeriamonocytogenesandcytoskeletalproteinsistheligandfortheEVH1domain,aproteinmodulepresentintheEna/VASPfamily:K.Niebuhr,etal.;EMBOJ.16,5433(1997)
- TheEVH2domainofthevasodilator-stimulatedphosphoproteinmediatestetramerization,F-actinbinding,andactinbundleformation:C.Bachmann,etal.;J.Biol.Chem.274,23549(1999)
- Vasodilator-StimulatedPhosphoproteinSerine239PhosphorylationasaSensitiveMonitorofDefectiveNitricOxide/cGMPSignalingandEndothelialDysfunction:M.Oelze,etal.;Circ.Res.87,999(2000)
- EffectsofInVivoNitroglycerinTreatmentonActivityandExpressionoftheGuanylylCyclaseandcGMP-DependentProteinKinaseandTheirDownstreamTargetVasodilator-StimulatedPhosphoproteininAorta:A.Muelsch,etal.;Circulation103,2188(2001)
- EffectsofangiotensinIIinfusionontheexpressionandfunctionofNAD(P)Hoxidaseandcomponentsofnitricoxide/cGMPsignaling:H.Mollnau,etal.;Circ.Res.90,E58(2002)
- Phosphorylationofbloodvesselvasodilator-stimulatedphosphoproteinatserine239asafunctionalbiochemicalmarkerofendothelialnitricoxide/cyclicGMPsignaling:C.Ibarra-Alvarado,etal.;MolPharmacol.61,312(2002)
- Changesofvasodilator-stimulatedphosphoprotein(VASP)anditsphosphorylationinendothelialcellsexposedtolaminarflow:L.Wei,etal.;Clin.Hemorheol.Microcirc.28,113(2003)
- Linkedregulationofmotilityandintegrinfunctioninactivatedmigratingneutrophilsrevealedbyinterferenceinremodellingofthecytoskeleton:S.I.Anderson,etal.;CellMotil.Cytoskeleton54,135(2003)
- Monitoringofclopidogrelaction:comparisonofmethods:J.Geiger,etal.;Clin.Chem.51,957(2005)
- VASP-dependentregulationofactincytoskeletonrigidity,celladhesion,anddetachment:A.B.Galler,etal.;Histochem.CellBiol.125,457(2006)
- AMP-activatedproteinkinaseimpairsendothelialactincytoskeletonassemblybyphosphorylatingvasodilator-stimulatedphosphoprotein:C.Blume,etal.;J.Biol.Chem.282,4601(2007)
- Cytoskeletonassemblyatendothelialcell-cellcontactsisregulatedbyαII-spectrin-VASPcomplexes:P.M.Benz,etal.;J.Cell.Biol.180,205(2008)
- PromotionofPDGF-inducedendothelialcellmigrationbyphosphorylatedVASPdependsonPKAanchoringviaAKAP:D.Zhang,etal.;Mol.Cell.Biochem.335,1(2010)
- Aproteinphosphorylation-basedassayforscreeningandmonitoringofdrugsmodulatingcyclicnucleotidepathways:J.Geiger,etal.;Anal.Biochem.407,261(2010)
- Apotentialroleforα-actininininside-outαIIbβ3signaling:S.Tadokoro,etal.;Blood117,250(2011)
- Proteomicsignatureofthrombin-activatedplateletsafterinvivonitricoxide-donortreatment:coordinatedinhibitionofsignaling(phosphatidylinositol3-kinase-γ,14-3-3ζ,andgrowthfactorreceptor-boundprotein2)andcytoskeletonproteintranslocation:E.Pena,etal.;Arterioscler.Thromb.Vasc.Biol.31,2560(2011)
- MonomericC-reactiveproteinisprothromboticanddissociatesfromcirculatingpentamericC-reactiveproteinonadheredactivatedplateletsunderflow:B.Molins,etal.;Cardiovasc.Res.92,328(2011)
品牌介绍
Adipogen公司由三个子公司组成，分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国，运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发，如癌症、免疫、炎症、代谢疾病（糖尿病、肥胖）、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外，也可提供抗体和蛋白产品，Adipogen拥有自己的化学实验室，致力于开发创新型小分子化合物。
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