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Functional_antibody >
Adipogen/anti-TIE-2 (human), mAb (tek9)/AG-20T-0103-C100/100 µg
| ProductDetails | |
|---|---|
| Synonyms | TyrosineKinasewithIgandEGFHomologyDomains-2;Angiopoietin-1Receptor |
| ProductType | MonoclonalAntibody |
| Properties | |
| Clone | tek9 |
| Isotype | MouseIgG1 |
| Immunogen/Antigen | RecombinanthumansolubleextracellulardomainofTIE-2. |
| Application | ELISA:(1-2μg/ml) FlowCytometry:(2-5μg/ml) Immunohistochemistry:(frozensection) WesternBlot:(2-5μg/ml) Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| Crossreactivity | Human |
| Specificity | NativehumanTIE-2inELISAexperimentsandonthesurfaceofdifferenthumancelltypes. |
| Purity | ProteinGpurified. |
| PurityDetail | ProteinGpurified. |
| Formulation | Lyophilized. |
| Reconstitution | Centrifugevialpriortoopening.Reconstitutewithsterilewatertoaconcentrationof0.1-1.0mg/ml. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Afteropening,preparealiquotsandstoreat-20°C. Avoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast6monthsafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
TIE-2actsascell-surfacereceptorforANGPT1,ANGPT2andANGPT4andregulatesangiogenesis,endothelialcellsurvival,proliferation,migration,adhesionandcellspreADIng,reorganizationoftheactinCytoskeleton,butalsomaintenanceofvascularquiescence.Ithasanti-inflammatoryeffectsbypreventingtheleakageofproinflammatoryplasmaproteinsandleukocytesfrombloodvessels.Itisrequiredfornormalangiogenesisandheartdevelopmentduringembryogenesisandforpost-natalhematopoiesis.Afterbirth,itactivatesorinhibitsangiogenesis,dependingonthecontext.Itinhibitsangiogenesisandpromotesvascularstabilityinquiescentvessels,whereendothelialcellshavetightcontacts.Inmigratingendothelialcellsthatlackcell-celladhesions,ANGT1recruitsTEKtocontactswiththeextracellularmatrix,leadingtotheformationoffocaladhesioncomplexes,activationofPTK2/FAKandofthedownstreamkinasesMAPK1/ERK2andMAPK3/ERK1andultimatelytothestimulationofsproutingangiogenesis.
- IdentificationofproangiogenicTIE2-expressingmonocytes(TEMs)inhumanperipheralbloodandcancer:M.A.Venneri,etal.;Blood109,5276(2007)
- Angiopoietin-2StimulationofEndothelialCellsInducesIntegrinαvβ3InternalizationandDegradation:M.Thomas,etal.;J.Biol.Chem.285,23842(2010)
