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Adipogen/anti-Polyglutamylation Modification, mAb (GT335)/AG-20B-0020-C100/100 µg
Adipogen/anti-Polyglutamylation Modification, mAb (GT335)/AG-20B-0020-C100/100 µg
商品编号：
AG-20B-0020-C100
品牌：
Adipogen Inc
市场价：
¥7900.00
美元价：
4740.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductDetails | |
|---|---|
| Synonyms | PolyglutamylatedTubulin;GlutamylatedTubulin;PostranslationalProteinGlutamylation |
| ProductType | MonoclonalAntibody |
| Properties | |
| Clone | GT335 |
| Isotype | MouseIgG1κ |
| Source/Host | Purifiedfromconcentratedhybridomatissueculturesupernatant. |
| Immunogen/Antigen | OctapeptideEGEGE*EEG,modifiedbytheadditionoftwoglutamylunitsontothefifthE(indicatedbyanasterisk). |
| Application | ElectronMicroscopy(seeLit.3) Immunocytochemistry:(1:2000) Immunohistochemistry:(paraffinsections;1:1000) Immunoprecipitation WesternBlot:(1:4000) Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| Crossreactivity | All |
| Specificity | Recognizestheposttranslationalmodification(poly)glutamylationonproteins.Reactswithpolyglutamylatedα-andβ-tubulin. |
| Purity | ≥95%(SDS-PAGE) |
| PurityDetail | ProteinG-affinitypurified. |
| Concentration | 1mg/ml |
| Formulation | Liquid.InPBScontaining10%glyceroland0.02%sodiumazide. |
| OtherProductData | Recognizesmostformsofpolyglutamylatedtubulin(α-andβ-tubulin),independentofthelengthoftheglutamatesidechains.Nospecificitytoparticulartubulinisoformsnortotubulinfromparticularspeciesareobserved.Detectsalsoother(poly)glutamylatedproteins.Sincenoconsensusmodificationsiteisknownforprotein(poly)glutamylation,thedetectionisnotsequence-specific.However,anacidicenvironmentofthemodificationsiteisrequired. Theuseoftheantibodyattoohighconcentrationsobscuresitsspecificityinimmunofluorescence. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Afteropening,preparealiquotsandstoreat-20°C. Avoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Polyglutamylationisapost-translationalmodificationinwhichglutamatesidechainsofvariablelengthsareformedonthemodifiedprotein.Itisevolutionarilyconservedandthemostprominentsubstrateistubulin,themicrotubule(MT)buildingblock.PolyglutamylationhasbeenproposedtobeinvolvedinthefunctionaladaptationofMTs,asitoccurswithinthecarboxy-terminaltubulintailsthatparticipatedirectlyinthebindingofmanystructuralandmotorMT-associatedproteins.Therecentidentificationofnewsubstratesofpolyglutamylationindicatesthatthispost-translationalmodificationcouldbeapotentialregulatorofdiversecellularprocessesandbeinvolvedincellcycleandcellproliferation.
ProductReferences
- Distributionofglutamylatedalphaandbeta-tubulininmousetissuesusingaspecificmonoclonalantibody,GT335:A.Wolff,etal.;Eur.J.CellBiol.59,425(1992)
- Polyglutamylationofnucleosomeassemblyproteins:C.Regnard,etal.;J.Biol.Chem.275,15969(2000)
- Glutamylatedtubulin:diversityofexpressionanddistributionofisoforms:M.L.Kann,etal.;CellMotil.Cytoskeleton55,14(2003)
- PolyglutamylationIsaPost-translationalModificationwithaBroadRangeofSubstrates:J.vanDijk,etal.;J.Biol.Chem.283,3915(2008)
- Uniquepost-translationalmodificationsinspecializedmicrotubulearchitecture:K.Ikegami&M.Setou;CellStruct.Funct.35,15(2010)(Review)
- Tubulindetyrosinationpromotesmonolayerformationandapicaltraffickinginepithelialcells:S.Zink|etal.;J.CellSci.125,5998(2012)
- TheCP110-interactingproteinsTalpid3andCep290playoverlappinganddistinctrolesinciliaassembly:T.Kobayashi,etal.;J.CellBiol.204,215(2014)
- CNSaxonsgloballyincreaseaxonaltransportafterperipheralconditioning:F.M.Mar,etal.;J.Neurosci.34,5965(2014)
- Microtubuledetyrosinationguideschromosomesduringmitosis:M.Barisic,etal.;Science348,6236(2015)(Supplement)
- SAS-6engineeringrevealsinterdependencebetweencartwheelandmicrotubulesindeterminingcentriolearchitecture:M.Hilbert,etal.;Nat.CellBiol.18,393(2016)
- LossofRPGRglutamylationunderliesthepathogenicmechanismofretinaldystrophycausedbyTTLL5mutations:X.Sun,etal.;PNAS113,E2925(2016)
- Alterationsinthebalanceoftubulinglycylationandglutamylationinphotoreceptorsleadstoretinaldegeneration:M.BoschGrau,etal.;J.Cell.Sci.130,938(2017)
- Theactin-MRTF-SRFtranscriptionalcircuitcontrolstubulinacetylationviaα-TAT1geneexpression:J.Fernández-Barrera,etal.;J.CellBiol.aheadofprint(2018)
品牌介绍
Adipogen公司由三个子公司组成，分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国，运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发，如癌症、免疫、炎症、代谢疾病（糖尿病、肥胖）、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外，也可提供抗体和蛋白产品，Adipogen拥有自己的化学实验室，致力于开发创新型小分子化合物。
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