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Adipogen/anti-Poly(ADP-ribose) [PAR], mAb (10H)/AG-20T-0001-C050/50 µg
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Adipogen/anti-Poly(ADP-ribose) [PAR], mAb (10H)/AG-20T-0001-C050/50 µg
商品编号：
AG-20T-0001-C050
品牌：
Adipogen Inc
市场价：
¥4200.00
美元价：
2520.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductDetails | |
|---|---|
| ProductType | MonoclonalAntibody |
| Properties | |
| Clone | 10H |
| Isotype | MouseIgG3κ |
| Source/Host | Purifiedfromconcentratedhybridomatissueculturesupernatant. |
| Immunogen/Antigen | Purifiedpoly(ADP-ribose). |
| Application | FlowCytometry:(seeA.Kunzmann,etal.;Immun.Ageing3,8(2006)) Immunocytochemistry:(5-20µg/ml) Immunohistochemistry(paraffinsections):Dilutein5%nonfatdriedmilkinPBStoafinalconcentrationof5-20µg/ml). WesternBlot:Incubate2.5µg/mlinPBS,0.05%Tween20,5%nonfatdriedmilk. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| Crossreactivity | Drosophila Human Mouse Rat |
| Specificity | Recognizespoly(ADP-ribose)synthesizedbyabroadrangeofPARPs(poly(ADP-ribose)polymerases),includinghuman,mouse,ratorDrosophilaPARPenzymes. |
| Purity | ≥95%(SDS-PAGE) |
| PurityDetail | ProteinA-affinitypurified. |
| EndotoxinContent | |
| Concentration | 1mg/ml |
| Formulation | Liquid.Containing50mMHEPES,pH7.4,100mMNaCl,1%BSAand0.02%sodiumazide. |
| OtherProductData | ApplicationNotes:Themonoclonalantibody10Hisdirectedagainstpoly(ADP-ribose)(PAR).AftermassiveDNAdamage(e.g.γ-irrADIationoroxidativestress)PARisdetectableinthefirst10minutesanddisappearslateron.Inkeratinocytestheanti-PAR(10H)hasbeenshowntodetectUVB-inducedapoptosisasearlyas4hoursafterirradiation,thusbeingsuperiortoDNAladderingandtheTUNELassay.Duetotheverylargenumberofendonuclease-mediatedDNAbreaksinapoptosis,PARP(poly(ADP-ribose)polymerase)becomesstronglyactivatedduringtheso-calledexecutionphase.InthecaseofDNAdamage-inducedapoptosis,thisrepresentsa"secondround"ofPARsynthesis.PARsynthesizedduringapoptosisappearstoberemarkablystable.PARimmunofluorescenceappearsatleastasearlyduringapoptosisasdoesthespecificcleavageofPARPbycaspase-3andcorrelateswellwithotherMarkersofapoptosis.anti-PAR(10H)wasusedinflowcytometryandaquantitativenon-isotopicimmuno-dot-blotmethodfortheassessmentofcellularpoly(ADP-ribosyl)ationcapacity. |
| ShippingandHandling | |
| Shipping | DRYICE |
| ShortTermStorage | -20°C |
| LongTermStorage | -80°C |
| HandlingAdvice | Afteropening,preparealiquotsandstoreat-20°C. Avoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-80°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Processessuchastranscription,repairandreplicationthatrequireefficientDNArecognitionaredependentonmodulationofchromatinstructure.ChromatinrelaxationisacriticaleventthatoccursduringDNArepairandisassociatedwiththenegativelychargedpolymerofadenosine5"-diphosphate(ADP)-ribose(PAR).PARissynthesizedfromnicotinamideadeninedinucleotide(NAD+)bythepoly(ADP-ribose)polymeraseproteinfamily(PARPs),ofwhichPARP-1(andtoalesserextentPARP-2)respondtoDNA-strandbreaks.PARP-1isselectivelyactivatedbyDNAstrandbreakstocatalyzetheadditionoflongbranchedchainsofPARtoavarietyofnuclearproteins,mostnotablyPARPitself.TheamountofPARformedinlivingcellswithDNAdamageiscommensuratewiththeextentofthedamage.UnderDNAdamageconditions,PARundergoesarapidturnover,withahalf-lifeintherangeofminutes,asPARisrapidlyhydrolyzedandconvertedtofreeADP-ribosebytheenzymepoly(ADP-ribose)glycohydrolase(PARG).PARregulatesnotonlycellsurvivalandcell-deathprogrammes,butalsoanincreasingnumberofotherBIOLOGicalfunctionswithwhichnovelmembersofthePARPfamilyhavebeenassociated.Theseincludetranscriptionalregulation,celldivision,intracellulartrafficking,inflammationandenergymetabolism.
ProductReferences
- Monoclonalantibodiestopoly(adenosinediphosphateribose)recognizedifferentstructures:H.Kawamitsu,etal.;Biochemistry23,3771(1984)
- Inhibitionofpoly(ADP-ribosyl)ationbyoverexpressingthepoly(ADP-ribose)polymeraseDNA-bindingdomaininmammaliancells:J.H.Kupperetal.;J.Biol.Chem.265,18721(1990)
- trans-dominantinhibitionofpoly(ADP-ribosyl)ationsensitizescellsagainstg-irradiationandN-methyl-N"-nitro-N-nitrosoguanidinebutdoesnotlimitDNAreplicationofapolyomavirusreplicon:J.H.Kuepper,etal.;Mol.Cell.Biol.15,3154(1995)
- Inactivationofthepoly(ADP-ribose)polymerasegeneaffectsoxygenradicalandnitricoxidetoxicityinisletcells:B.Heller,etal.;J.Biol.Chem.270,11176(1995)
- Poly(ADP-ribose)synthesis:ausefulparameterforidentifyingapoptoticcells:M.Donzelli,etal.;Histochem.J.29,831(1997)
- Multiparametricstainingtoidentifyapoptotichumancells:C.Negri,etal.;Exp.CellRes.234,174(1997)
- Poly(ADP-ribose)immunostainingtodetectapoptosisinducedbyaneurotoxicfragmentofprionprotein:A.Buerkle,etal.;Histochem.J.31,711(1999)
- Detectionofpoly(ADP-ribose)synthesisinDrosophilatestesupongamma-irradiation:S.Lankenau,etal.;Chromosoma108,44(1999)
- 4-Amino-1,8-naphthalimide:anovelinhibitorofpoly(ADP-ribose)polymeraseandradiationsensitizer:A.Schlicker,etal.;Int.J.Radiat.Biol.75,91(1999)
- Reactiveoxygenspeciesparticipateinmdr1bmRNAandP-glycoproteinoverexpressioninprimaryrathepatocytecultures:C.Ziemann,etal.;Carcinogenesis20,407(1999)
- Selectivelossofpoly(ADP-ribose)andthe85-kDafragmentofpoly(ADP-ribose)polymeraseinnucleoliduringalkylation-inducedapoptosisofHeLacells:R.Alvarez-Gonzalez,etal.;J.Biol.Chem.274,32122(1999)
- OverexpressionofdominantnegativePARPinterfereswithtumorformationofHeLacellsinnudemice:evidenceforincreasedtumorcellapoptosisinvivo:M.A.Hans,etal.;Oncogene18,7010(1999)
- Quantitativenonisotopicimmuno-dot-blotmethodfortheassessmentofcellularpoly(ADP-ribosyl)ationcapacity:R.Pfeiffer,etal.;Anal.Biochem.275,118(1999)(Immuno-Dot-BlotDetection)
- Poly(ADP-ribose)polymerasecleavageduringapoptosis:whenandwhere?:C.Soldani,etal.;Exp.CellRes.269,193(2001)
- ActivationandCaspase-mediatedInhibitionofPARP:AMolecularSwitchbetweenFibroblastNecrosisandApoptosisinDeathReceptorSignaling:M.Los,etal.;Mol.Biol.Cell.13,978(2002)
- Flow-cytometricassessmentofcellularpoly(ADP-ribosyl)ationcapacityinperipheralbloodlymphocytes:A.Kunzmann,etal.;Immun.Ageing3,8(2006)(FACS)
品牌介绍
Adipogen公司由三个子公司组成，分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国，运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发，如癌症、免疫、炎症、代谢疾病（糖尿病、肥胖）、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外，也可提供抗体和蛋白产品，Adipogen拥有自己的化学实验室，致力于开发创新型小分子化合物。
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