| ProductDetails | |
|---|---|
| Synonyms | Osteoblast-specificFactor2;OSF-2;Postn |
| ProductType | MonoclonalAntibody |
| Properties | |
| Clone | Stiny-1 |
| Isotype | MouseIgG1κ |
| Source/Host | Purifiedfromconcentratedhybridomatissueculturesupernatant. |
| Immunogen/Antigen | Full-lengthhumanperiostin. |
| Label/Conjugates | PreservativeFree |
| Application | ELISA Immunohistochemistry:(1:200-1:500;paraffinsectionsandfrozensections) WesternBlot:(1:1"000) ForFunctionalApplicationuse:anti-Periostin,mAb(blocking)(OC-20)(AG-20B-6000PF) |
| Crossreactivity | Human Mouse |
| Specificity | Recognizeshumanandmouseperiostin. |
| Purity | ≥95%(SDS-PAGE) |
| PurityDetail | ProteinG-affinitypurified. |
| Concentration | 1mg/ml |
| Formulation | Liquid.InPBScontaining10%glycerol. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Afteropening,preparealiquotsandstoreat-20°C. Avoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Periostinisa90-kDamatricellularproteinthatconsistsofatypicalsignalsequence,followedbyacysteine-richregion,anEMIdomain(protein-proteininteractions),fourtandemfasciclin-likedomainsthatareresponsIBLeforintegrinbinding,andaC-terminalregion.Periostinwasoriginallyisolatedasanosteoblast-specificfactorthatfunctionsasacelladhesionmoleculeforpre-osteoblastsandinosteoblastrecruitment,attachmentandspreADIng.PeriostinisalsoinvolvedinmanyfundamentalBIOLOGicalprocessessuchascellproliferation,cellinvasionandangiogenesis.Periostinexpressionisincreasedbybothtransforminggrowthfactorβ1(TGF-β1)andbonemorphogeneticprotein2(BMP-2).Changesinperiostinexpressionarecommonlydetectedinvariouscancersandpre-cancerousconditions,andperiostinmaybeinvolvedinregulatingcancercellactivitiesthatcontributetotumOrigenesis,cancerprogressionandmetastasis.Periostinhasshowntobeinvolvedinmanyaspectsofallergicinflammation,suchaseosinophilrecruitment,airwayremodeling,developmentofaTh2phenotypeandincreasedexpressionofinflammatorymediators.ItisevaluatedasabioMarkerforbronchialasthmaandairwayinflammation.
- Interactionsbetweencancerstemcellsandtheirnichegovernmetastaticcolonization:I.Malanchi,etal.;Nature481,85(2012)
- Periostincontributestotheacquisitionofmultipotentstemcell-likepropertiesinhumanmammaryepithelialcellsandbreastcancercells:X.Wang,etal.;PLoSOne8,e72962(2013)
- Theperivascularnicheregulatesbreasttumourdormancy:C.M.Ghajar,etal.;Nat.CellBiol.15,807-817(2013)
- Matricellularproteinperiostincontributestohepaticinflammationandfibrosis:Y.Huang,etal.;Am.J.Pathol.185,786(2015)
- Endogenoussemaphorin-7Aimpedeshumanlungfibroblastdifferentiation:S.Esnault,etal.;PLoSOne12,e0170207(2017)
