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Adipogen/anti-Myosin IIA (non-muscle) (heavy chain), mAb (rec.) (SF9) (ATTO 488)/AG-27B-0010TD-C100/100 µg
| ProductDetails | |
|---|---|
| Synonyms | CellularMyosinHeavyChain,TypeA;MyosinHeavyChain9;MyosinHeavyChain,Non-muscleIIa;Non-muscleMyosinHeavyChainA |
| ProductType | RecombinantAntibody |
| Properties | |
| Clone | SF9 |
| Isotype | HumanIgG2&lamBDa; |
| Source/Host | Purifiedfrom HEK293cell culturesupernatant. |
| Immunogen/Antigen | FulllengthmyosinIIAfromratliver. |
| Label/Conjugates | ATTO488 |
| Application | Immunocytochemistry:(1:1000) |
| Crossreactivity | Drosophila Human Mouse Rat |
| Specificity | Recognizeshuman,mouse,ratanddrosophilamyosinIIA(heavychain). |
| Purity | ≥95%(SDS-PAGE) |
| PurityDetail | ProteinA-affinitypurified. |
| Concentration | 1mg/ml |
| Formulation | Liquid.InPBScontaining10%glyceroland0.02%sodiumazide. |
| OtherProductData | anti-MyosinIIA(non-muscle),monoclonalantibody(recombinant)(SF9)(ATTO488)iscomposedofhumanvariableregions(VHandVL)(λ-chain)ofimmunoglobulinfusedtothehumanlgG2FcdomainandlabeledwithATTO488. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | +4°C |
| HandlingAdvice | Avoidfreeze/thawcycles. |
| Use/StABIlity | Keepconjugatedformatsat+4°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
anti-MyosinIIA(non-muscle),monoclonalantibody(recombinant)(SF9)isanantibodydevelopedbyantibodyphagedisplaytechnologyusingahumannaiveantibodygenelibrary.TheselibrariesconsistofscFv(singlechainfragmentvariable)composedofVH(variabledomainofthehumanimmunoglobulinheavychain)andVL(variabledomainofthehumanimmunoglobulinlightchain)connectedbyapolypeptidelinker.Theantibodyfragmentsaredisplayedonthesurfaceoffilamentousbacteriophage(M13).ThisscFvwasselectedbyaffinityselectiononantigeninaprocesstermedpanning.Multipleroundsofpanningareperformedtoenrichforantigen-specificscFv-phage.Monoclonalantibodiesaresubsequentlyidentifiedbyscreeningaftereachroundofselection.TheselectedmonoclonalscFvisclonedintoanappropriatevectorcontainingaFcportionofinterestandthenproducedinmammaliancellstogenerateanIgGlikescFv-Fcfusionprotein.
- Recombinantantibodiesselectedagainstsubcellularfractionstotrackendogenousproteindynamicsinvivo:C.Nizak,etal.;Traffic7,739(2003)
