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Adipogen/anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free)/AG-27B-0016PF-M001/1 mg
| ProductDetails | |
|---|---|
| Synonyms | Ang-2;Ang2;Angpt2;Agpt2 |
| ProductType | RecombinantAntibody |
| Properties | |
| Clone | Angy-2-1 |
| Isotype | MouseIgG2b&lamBDa;. |
| Source/Host | CHOcells |
| Immunogen/Antigen | Recombinanthumanangiopoietin-2. |
| Label/Conjugates | PreservativeFree |
| Application | ELISA |
| Crossreactivity | Human Mouse |
| Specificity | Recognizeshumanandmouseangiopoietin-2.Doesnotdetecthumanangiopoietin-1. |
| Purity | ≥95%(SDS-PAGE) |
| PurityDetail | ProteinA-affinitypurified. |
| Concentration | 1mg/ml |
| Formulation | Liquid.InPBScontaining10%glycerol. |
| OtherProductData | anti-Angiopoietin-2,mAb(rec.)(blocking)(Angy-2-1)(preservativefree)iscomposedofhumanvariableregions(VHandVL)(λ-chain)ofimmunoglobulinfusedtothemouselgG2bFcdomain. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Afteropening,preparealiquotsandstoreat-20°C. Avoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Angiopoietin-1(Ang-1)andAngiopoietin-2(Ang-2)arecloselyrelatedsecretedligandswhichbindwithsimilaraffinitytoTie-2.Tie-2andangiopoietinshavebeenshowntoplaycriticalrolesinembryogenicangiogenesisandinmaintainingtheintegrityoftheadultvasculature.Ang-1activatesTie-2signalingonendothelialcellstopromotechemotaxis,cellsurvival,cellsprouting,vesselgrowthandstabilization.Ang-2hasbeenidentifiedasasecretedproteinligandofTie-2andhasalternativelybeenreportedtobeanantagoNISTforAng-1inducedTie-2signalingaswellasanagonistforTie-2signaling,dependingonthecellcontext.
anti-Angiopoietin-2,mAb(rec.)(blocking)(Angy-2-1)(preservativefree)isanantibodydevelopedbyantibodyphagedisplaytechnologyusingahumannaiveantibodygenelibrary.TheselibrariesconsistofscFv(singlechainfragmentvariable)composedofVH(variabledomainofthehumanimmunoglobulinheavychain)andVL(variabledomainofthehumanimmunoglobulinlightchain)connectedbyapolypeptidelinker.Theantibodyfragmentsaredisplayedonthesurfaceoffilamentousbacteriophage(M13).ThisscFvwasselectedbyaffinityselectiononantigeninaprocesstermedpanning.Multipleroundsofpanningareperformedtoenrichforantigen-specificscFv-phage.Monoclonalantibodiesaresubsequentlyidentifiedbyscreeningaftereachroundofselection.TheselectedmonoclonalscFvisclonedintoanappropriatevectorcontainingaFcportionofinterestandthenproducedinmammaliancellstogenerateanIgGlikescFv-Fcfusionprotein.
- Elevatedangiopoietin-2levelinpatientswithcontinuous-flowleftventricularassistdevicesleadstoalteredangiogenesisandisassociatedwithhighernonsurgicalbleeding:C.E.Tabit,etal.;Circulation134,141(2016)
- Angiopoietin2inducesastrocyteapoptosisviaαvβ5-integrinsignalingindiabeticretinopathy:J.H.Yun,etal.;CellDeathDis.7,e2101(2016)
- Angiopoietin-2inwhiteADIposetissueimprovesmetabolichomeostasisthroughenhancedangiogenesis:eLife6,e24071(2017)
