脂肪原/双泛素[Ub2](K48连接)(人类)(受体)(TAMRA Pos2)/AG-40T-0239-C050/50µg
商品编号:
AG-40T-0239-C050
品牌:
Adipogen Inc
市场价:
¥8740.00
美元价:
5244.00
产品分类:
临床研究
公司分类:
clinical_research
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
ProductDetails | |
---|---|
ProductType | Protein |
Properties | |
Source/Host | E.coli |
Sequence | Twohumanubiquitin(AccessionNr.P0CG47)covalentlylinkedthroughanisopeptidebondatK48residueofoneubiquitinmoleculeandtheC-terminalglycineresidueofanotherubiquitinmoleculeandconjugatedtoTAMRA. |
Crossreactivity | Human |
Label/Conjugates | TAMRA |
Formulation | Liquid.In50mMHepespH7.5,150mMNaCl,2mMDTT. |
OtherProductData | Use:Linkage-specificdi-ubiquitinisasubstrateforenzymesthatcleavetheisopeptidelinkagebetweentwoubiquitinmolecules.ThisFRET-basedDUBsubstratewithisopeptidebondsbetweenubiquitinmoietiesissuperiortogeldensitometryassays.SinceDUBsrecognizeandcleavesubstrateswithspecificstericconditions,eachsubstratevariesinthepositionofthefluorophoreandquencherandmustbeempiricallytestedforindividualenzymes.Idealforuseasasubstrateforubiquitin-specifichydrolaseswithactivitytowardK48linkages.Werecommendanassaybufferconsistingof50mMHepespH7.5,150mMNaCl,2mMDTT.Fluorescencecanbemonitoredwithanexcitationwavelengthof544nMandanemissionwavelengthof572nM.Reactionconditionswillneedtobeoptimizedforeachspecificapplication.Werecommendaninitialproteinconcentrationof100-500nM. |
Declaration | ManufacturedbyBostonBiochem |
ShippingandHandling | |
Shipping | DRYICE |
ShortTermStorage | -20°C |
LongTermStorage | -80°C |
HandlingAdvice | Aliquottoavoidfreeze/thawcycles. Protectfromlight. |
Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-80°C. |
Documents | |
MSDS | No |
ProductSpecificationSheet | |
Datasheet | DownloadPDF |
Withapredictedmolecularweightof17kDa,di-ubiquitiniscomposedoftwoubiquitinmonomersthatarecovalentlylinkedthroughanisopeptidebond,whichtypicallyformbetweenalysineresidueofoneubiquitinmoleculeandtheC-terminalglycineresidueofanotherubiquitinmolecule.Eachhumanubiquitinmonomeris76aminoacids(aa)inlengthandshares96%and100%aaidentitywithyeastandmouseubiquitin,respectively.Ubiquitinhassevenlysineresiduesthatcanparticipateintheformationofpoly-ubiquitinchains.Thespecificlysineresidueusedinubiquitinconjugationisthoughttodeterminethefunctionofpoly-ubiquitinationincellularprocessessuchasproteindegradation,signalingandtrafficking.
品牌介绍
Adipogen公司由三个子公司组成,分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国,运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发,如癌症、免疫、炎症、代谢疾病(糖尿病、肥胖)、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外,也可提供抗体和蛋白产品,Adipogen拥有自己的化学实验室,致力于开发创新型小分子化合物。
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