Adipogen/ITCH E3 Ligase TR-FRET Kit/SBB-KF0035-KI01/1 Kit
商品编号：
SBB-KF0035-KI01
品牌：
Adipogen Inc
市场价：
¥13220.00
美元价：
7932.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductDetails | |
|---|---|
| Synonyms | AIF4;AIP4;NAPP1 |
| ProductType | Kit |
| Properties | |
| ApplicationSet | CompoundScreening |
| Specificity | Readout:Endpoint/Kinetic LabelorDye:Europium-Cryptate&Cy5 DetectionMethod:TR-FRET SubstrateProperties:Protein-basedSubstrate.Typicalexperimentalconcentrationis1x. Excitation/Emission(nm):304/620&304/665 |
| Crossreactivity | Human |
| Quantity | SufficientReagentsfor: 400x20μlreactions 160x50μlreactions |
| KitContains | 100xUBA1(100μl) 100xUBE2L3(100μl) 100xITCH(100μl) 100xTRF-UbiquitinMix(100μl) 10xReactionBuffer(1ml) 10xMg-ATP(1ml) 1x384-wellwhitelow-volumemicroplate |
| OtherProductData | ThekitusesubiquitinlabeledwitheitherEuropium-CryptateorCy5asFRETpairdonorandacceptorfluorophoresrespectively,completelyeliminatingtheneedforantibodybaseddetectionsetups.EnzymaticincorporationofthelabeledubiquitinsintochainsconjugatedontoITCHleadstoanincreaseinfluorescenceemissionat665nm(Em-Acceptor)anddecreaseatemissionwavelength620nm(Em-Donor). ClickhereforaTypicalLot-specificProductDatasheetfromtheOriginalManufacturer Ourproductdescriptionmaydifferslightlyfromtheoriginalmanufacturersproductdatasheet. |
| Declaration | ManufacturedbySouthBayBio. |
| ShippingandHandling | |
| Shipping | DRYICE |
| ShortTermStorage | -20°C |
| LongTermStorage | -80°C |
| HandlingAdvice | Aliquottoavoidfreeze/thawcycles. Avoidfreeze/thawcycles. |
| Documents | |
| Manual | No |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet |  DownloadPDF |
SouthBayBio’sITCHTR-FRETUbiquitinKitprovidesafastandsensitivemethodmonitoringubiquitinconjugationontoITCHinsolution,resultingfromanenzymaticubiquitincascadewithouttheneedofrunningandstaininganSDSgel.ThekitenablescontinuousTR-FRETdetectionofubiquitinchainformationontoITCHinareal-timedetectionsetup,orinanend-pointconfigurationifdesired.TR-FRETusestheextendedfluorescenceemissiondecaylifetimestypicalofrare-earthlanthanidestoimpartashorttime-delaybetweenFRETdonorexcitationandemission.Thisdelayprovidesameanstoseparate“true”signalfromshort-livedbackgroundfluorescence,andreduceinterferencefromcompoundfluorescenceandotherassayartifacts.
ProductReferences
- Real-TimeHomogenousTR-FRETUbiquitinConjugationandDeconjugationAssayPlatform:I.Tomasic:Poster,EMBOConferenceUbiquitinandSUMO,Cavtat-Dubrovnik(2017)
品牌介绍
Adipogen公司由三个子公司组成，分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国，运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发，如癌症、免疫、炎症、代谢疾病（糖尿病、肥胖）、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外，也可提供抗体和蛋白产品，Adipogen拥有自己的化学实验室，致力于开发创新型小分子化合物。
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