Specifications&Handling:

ProductData
Synonyms	SmallUbiquitin-relatedModifier3;HSMT3;SMT3Homolog1;Ubiquitin-likeProteinSMT3B
Properties
Crossreactivity	Human
KitContains	20µlof10XSUMOE1Enzyme 20µlof10XSUMO3 20µlof10XUbc9(UBE2I) 20µlof10XReactionBuffer 20µlof10XMg-ATPSolution NOTE:Kitcontainsreagentssufficientfor5-10x20μlreactions.
OtherProductData	Use:Thiskitisdesignedfortheconjugationoftheubiquitin-likemodifierSUMO-3toproteinsubstratesinvitro,whichrequirestheactivitiesoftheheterodimerichumanE1activatingenzyme(SAE1/SAE2)andtheUbc9E2enzyme.
Origin	ManufacturedbyBostonBiochem
ProductType	Kit
ShippingandHandling
Shipping	DRYICE
ShortTermStorage	-20°C
LongTermStorage	-80°C
HandlingAdvice	Aliquottoavoidfreeze/thawcycles.
Use/StABIlity	Stableforatleast1yearafterreceiptwhenstoredat-80°C.
Documents
Manual	Download

Description:

Theubiquitin-likeSUMO-3isconjugatedtoavarietyofproteinsinthepresenceofUbc9andtheSUMOE1activatingenzyme(SAE1/SAE2inhuman,orAos1/Uba2pinyeast).Thisisoformisknowntoformpoly-SUMOchainsonsubstratesviaisopeptidebondformationbetweentheC-terminalglycineofoneSUMO-3tothelysine-ε-aminogroupofK11ontheprecedingSUMO-3.TheheterodimericSAE1/SAE2complex(38and70kDarespectively)usesATPtoadenylatetheC-terminalglycineresidueofSUMO-3,formingahigh-energythiolesterbondwiththeSAE2subunit.Thesecondstepisthetrans-esterificationreactionwherebytheactivatedSUMO-3istransferredtoCys93ofUbc9.Ubc9isamemberoftheE2familyandishomologoustoubiquitin-conjugatingenzymes,butisspecificfortheconjugationofSUMOtoavarietyoftargetproteins.ThisE2isunusualinthatitinteractsdirectlywithproteinsubstratesthataremodifiedbysumolyation,andmayplayaroleinsubstraterecognition.Sumoylatedsubstratesareprimarilylocalizedtothenucleus(RanGAP-1,RANBP2,PML,p53,Sp100,HIPK2)buttherearealsocytosolicsubstrates(IκBα,GLUT1,GLUT4).SUMOmodificationhasbeenimplicatedinfunctionssuchasnucleartransport,chromosomesegregationandtranscriptionalregulation,apoptosis,proteinfunctionandstability.

Adipogen公司由三个子公司组成，分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国，运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发，如癌症、免疫、炎症、代谢疾病（糖尿病、肥胖）、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外，也可提供抗体和蛋白产品，Adipogen拥有自己的化学实验室，致力于开发创新型小分子化合物。