脂肪原/SUMO2(人)结合试剂盒/AG-44T-0126-KI01/1试剂盒
商品编号:
AG-44T-0126-KI01
品牌:
Adipogen Inc
市场价:
¥12120.00
美元价:
7272.00
产品分类:
竞争法ELISA
公司分类:
Competition_law_ELISA
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Specifications&Handling:
ProductData | |
---|---|
Synonyms | SmallUbiquitin-relatedModifier2;HSMT3;SMT3Homolog2;Sentrin-2;Ubiquitin-likeProteinSMT3A |
Properties | |
Crossreactivity | Human |
KitContains | 20µlof10XSUMOE1Enzyme 20µlof10XSUMO2 20µlof10XUbc9(UBE2I) 20µlof10XReactionBuffer 20µlof10XMg-ATPSolution NOTE:Kitcontainsreagentssufficientfor5-10x20μlreactions. |
OtherProductData | Use:Thiskitisdesignedfortheconjugationoftheubiquitin-likemodifierSUMO-2toproteinsubstratesinvitro,whichrequirestheactivitiesoftheheterodimerichumanE1activatingenzyme(SAE1/SAE2)andtheUbc9E2enzyme. |
Origin | ManufacturedbyBostonBiochem |
ProductType | Kit |
ShippingandHandling | |
Shipping | DRYICE |
ShortTermStorage | -20°C |
LongTermStorage | -80°C |
HandlingAdvice | Aliquottoavoidfreeze/thawcycles. |
Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-80°C. |
Documents | |
Manual | Download |
Description:
Theubiquitin-likeSUMO-2isconjugatedtoavarietyofproteinsinthepresenceofUbc9andtheSUMOE1activatingenzyme(SAE1/SAE2inhuman,orAos1/Uba2pinyeast).Thisisoformisknowntoformpoly-SUMOchainsonsubstratesviaisopeptidebondformationbetweentheC-terminalglycineofoneSUMO-2tothelysine-ε-aminogroupofK11ontheprecedingSUMO-2.TheheterodimericSAE1/SAE2complex(38and70kDarespectively)usesATPtoadenylatetheC-terminalglycineresidueofSUMO-2,formingahigh-energythiolesterbondwiththeSAE2subunit.Thesecondstepisthetrans-esterificationreactionwherebytheactivatedSUMO-2istransferredtoCys93ofUbc9.Ubc9isamemberoftheE2familyandishomologoustoubiquitin-conjugatingenzymes,butisspecificfortheconjugationofSUMOtoavarietyoftargetproteins.ThisE2isunusualinthatitinteractsdirectlywithproteinsubstratesthataremodifiedbysumolyation,andmayplayaroleinsubstraterecognition.Sumoylatedsubstratesareprimarilylocalizedtothenucleus(RanGAP-1,RANBP2,PML,p53,Sp100,HIPK2)buttherearealsocytosolicsubstrates(IκBα,GLUT1,GLUT4).SUMOmodificationhasbeenimplicatedinfunctionssuchasnucleartransport,chromosomesegregationandtranscriptionalregulation,apoptosis,proteinfunctionandstability.品牌介绍
Adipogen公司由三个子公司组成,分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国,运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发,如癌症、免疫、炎症、代谢疾病(糖尿病、肥胖)、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外,也可提供抗体和蛋白产品,Adipogen拥有自己的化学实验室,致力于开发创新型小分子化合物。
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