脂肪原/Kdo2脂质A 96孔板(无菌)/AG-44T-0002-KI01/1板
商品编号:
AG-44T-0002-KI01
品牌:
Adipogen Inc
市场价:
¥5200.00
美元价:
3120.00
产品分类:
竞争法ELISA
公司分类:
Competition_law_ELISA
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Specifications&Handling:
ProductData | |
---|---|
Synonyms | Kdo2-LipidA-TLR4AgoNISTArray |
Properties | |
ApplicationSet | CompoundScreening |
Specificity | TLR4 |
OtherProductData | AdditionalStorageAdvise: Unopenedpouch:Ambient.Donotfreeze. Openedplatestoredinre-sealedpouch:4°C.Donotfreeze.Ifkeptsterile,theplate(withtheincludedlidattached)canbeusedforamultiplecustomizedexperimentalsetupswherecellculturesUSPensionscanbeaddedsequentiallyorindividualwellsusedforseparatetestdates. Alreadyusedwellsshouldbemarkedappropriatelyandanycellculturemediumaspiratedcompletelyundersterileconditions.Anypartiallyusedplateshouldbekeptsterileuntilfurtheruse. ClickhereforfullProductDatasheet. |
ProductType | Kit |
ShippingandHandling | |
Shipping | AMBIENT |
ShortTermStorage | +20°C |
LongTermStorage | +20°C |
HandlingAdvice | Donotfreeze. |
Use/StABIlity | Stableforatleast2yearsafterreceiptwhenstoredat+20°C. |
Documents | |
Manual | Download |
MSDS | Download |
RelatedProducts | |
Description:
Kdo2-LipidAplatescontainahighquality,sterileflat-bottomedclearpolystyrene96-wellcellcultureplatepre-coatedwithhighpurityLPSfromE.coli(Re)(Kdo2-LipidA).Theplatesarecoatedinauniform,stabilizedandactivelayerofpredefinedquantitiesofhighpurityKdo2-LipidAandtheyaresetupas12testcolumnswithidenticalagonistconcentrationsin7rows(A-G):RowConcentrations:1μg(A),100ng(B),10ng(C),1ng(D),100pg(E),10pg(F),1pg(G)perwellandonenegativecontrolrow(H).
ARRAYPROCEDURE:
AddprimarycellsorcelllinesexpressingTLR4totheKdo2-LipidAplatewellscontainingthecoatedTLR4-selectivehighpurityKdo2-LipidA.Optimalcelldensityneedstobedeterminedfortherespectivecelltypeandreadout,recommendedstartingconcentrationis50,000macrophagesperwell.RecommendedcellculturevolumetobeaddedtotheKdo2-LipidAplateis100μl(200μl)per96-well,correspondingtoastartconcentrationof10μg/ml(5μg/ml)perwellfollowedby10-folddilutionsto10pg/ml(5pg/ml).
APPLICATION&CONVENIENCE:
1.Kdo2-LipidAplatesfacilitatethedeterminationoftheoptimalcelldensity,timepointofanalysisandmultiplexingofreadouts(e.g.cytokine(s)ELISA)ofTLR4specificcellularactivationinahighlyreproducIBLeandsafeprocedureexcludingthepossibilityofcontaminationorhazardoushandlingofKdo2-LipidA.
2.Kdo2-LipidAplatesareusefulforestablishingadoseresponsecurvespecificforthechosencelltypeandserumconcentrationandfortestingofTLR4inhibitorsorsensitizers.
3.Thisisaconvenientandtimesavingformatthathelpstoavoiddilutionerrors,partialsolubilisationorcontaminationwithKdo2-LipidA.
品牌介绍
Adipogen公司由三个子公司组成,分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国,运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发,如癌症、免疫、炎症、代谢疾病(糖尿病、肥胖)、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外,也可提供抗体和蛋白产品,Adipogen拥有自己的化学实验室,致力于开发创新型小分子化合物。
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